Description
ZKSCAN3 Antibody (PACO50986)
The ZKSCAN3 Polyclonal Antibody (PAC050986) is a valuable tool for researchers studying ZKSCAN3, a transcriptional repressor involved in various cellular processes. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By specifically binding to the ZKSCAN3 protein, this antibody allows for precise detection and analysis in a range of cell types, making it well-suited for investigations in molecular biology and disease research.ZKSCAN3, also known as zinc finger with KRAB and SCAN domains 3, is a key regulatory factor that modulates gene expression and plays a crucial role in cell proliferation and differentiation.
Its involvement in diverse biological pathways highlights its potential importance in conditions such as cancer, developmental disorders, and neurological diseases. By studying ZKSCAN3 activity, researchers can gain insights into its function and explore therapeutic strategies targeting this molecule for intervention in various health conditions.
| Antibody Name: | ZKSCAN3 Antibody (PACO50986) |
| Antibody SKU: | PACO50986 |
| Size: | 50ug |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, WB, IHC, IF |
| Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200 |
| Species Reactivity: | Human, Mouse |
| Immunogen: | Recombinant Human Zinc finger protein with KRAB and SCAN domains 3 protein (8-275AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | Western Blot. Positive WB detected in: Mouse heart tissue, Mouse spleen tissue, Mouse lung tissue. All lanes: ZKSCAN3 antibody at 4µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. predicted band size: 61, 44 kDa. observed band size: 61, 44 kDa.. |
 | Immunofluorescent analysis of Hela cells using PACO50986 at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
 | Immunohistochemistry of paraffin-embedded human lymph node tissue using PACO50986 at dilution of 1:100. |
| Background: | Transcriptional factor that binds to the consensus sequence 5'-[GT][AG][AGT]GGGG-3' and acts as a repressor of autophagy. Specifically represses expression of genes involved in autophagy and lysosome biogenesis/function such as MAP1LC3B, ULK1 or WIPI2. Associates with chromatin at the ITGB4 and VEGF promoters. Also acts as a transcription activator and promotes cancer cell progression and/or migation in various tumors and myelomas. |
| Synonyms: | Zinc finger protein with KRAB and SCAN domains 3 (Zinc finger and SCAN domain-containing protein 13) (Zinc finger protein 306) (Zinc finger protein 309) (Zinc finger protein 47 homolog) (Zf47) (Zfp-47), ZKSCAN3, ZFP47 ZNF306 ZNF309 ZSCAN13 |
| UniProt Protein Function: | ZNF306: Acts as a transcriptional regulator. Binds to the consensus sequence 5'-[GT][AG][AGT]GGGG-3'. Associates with chromatin at the ITGB4 and VEGF promoters. Activates the transcription of genes associated with colon cancer progression. Belongs to the krueppel C2H2-type zinc-finger protein family. |
| UniProt Protein Details: | Protein type:Transcription factor; DNA-binding; C2H2-type zinc finger protein Chromosomal Location of Human Ortholog: 6p22.1 Cellular Component: cytoplasm; nucleus Molecular Function:chromatin binding; DNA binding; protein binding; sequence-specific DNA binding; transcription factor activity Biological Process: lysosome organization and biogenesis; negative regulation of autophagy; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; positive regulation of transcription, DNA-dependent |
| UniProt Code: | Q9BRR0 |
| NCBI GenInfo Identifier: | 116242859 |
| NCBI Gene ID: | 80317 |
| NCBI Accession: | Q9BRR0.2 |
| UniProt Secondary Accession: | Q9BRR0,Q5VXH3, Q92972, Q9H4T3, B2R8W2, B3KVC0, H7BXX1 |
| UniProt Related Accession: | Q9BRR0 |
| Molecular Weight: | 43,793 Da |
| NCBI Full Name: | Zinc finger protein with KRAB and SCAN domains 3 |
| NCBI Synonym Full Names: | zinc finger with KRAB and SCAN domains 3 |
| NCBI Official Symbol: | ZKSCAN3Â Â |
| NCBI Official Synonym Symbols: | ZF47; Zfp47; ZFP306; ZNF306; ZNF309; zfp-47; ZSCAN13; ZSCAN35; dJ874C20.1; dJ874C20.1.  |
| NCBI Protein Information: | zinc finger protein with KRAB and SCAN domains 3 |
| UniProt Protein Name: | Zinc finger protein with KRAB and SCAN domains 3 |
| UniProt Synonym Protein Names: | Zinc finger and SCAN domain-containing protein 13; Zinc finger protein 306; Zinc finger protein 309; Zinc finger protein 47 homolog; Zf47; Zfp-47 |
| Protein Family: | Zinc finger protein with KRAB and SCAN domains |
| UniProt Gene Name: | ZKSCAN3Â Â |
| UniProt Entry Name: | ZKSC3_HUMAN |
