The ZNF177 Antibody (CAB14803) is a high-quality antibody developed for reliable detection and analysis of target proteins. Enables sequence-specific double-stranded DNA binding activity. Predicted to be involved in negative regulation of transcription by RNA polymerase II. Located in blood microparticle.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Mouse samples.
Product Name:
ZNF177 Antibody
SKU:
CAB14803
Size:
100μL, 20μL
Reactivity:
Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:2000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
PIGX, ZNF177
Positive Sample:
Mouse testis
Cellular Localization:
Nucleus.
Calculated MW:
55kDa
Observed MW:
55kDa
Enables sequence-specific double-stranded DNA binding activity. Predicted to be involved in negative regulation of transcription by RNA polymerase II. Located in blood microparticle.
Purification Method
Affinity purification
Gene ID
7730
RRID
AB_2761681
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from mouse testis, using ZNF177 Rabbit pAb (CAB14803) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 2.5min.
