The ZNF397 Polyclonal Antibody (PAC021899) is a valuable tool for researchers studying the zinc finger protein ZNF397. This antibody, raised in rabbits, is highly specific and reactive with human samples, making it suitable for a variety of research applications.ZNF397 is a transcription factor that plays a critical role in gene regulation and is implicated in various cellular processes, including proliferation, differentiation, and development. It is also involved in the regulation of immune responses and has been linked to certain disease pathways.
This antibody enables detection and analysis of ZNF397 in different cell types, making it ideal for studies in molecular biology, genetics, and immunology. Its validation for use in techniques such as Western blotting ensures reliable and accurate results, contributing to the advancement of scientific knowledge in these areas. Explore the functions and mechanisms of ZNF397 with the precision and reliability of the PAC021899 antibody.
Antibody Name:
ZNF397 Antibody (PACO21899)
Antibody SKU:
PACO21899
Size:
100ul
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Recommended Dilutions:
ELISA:1:2000-1:10000, WB:1:500-1:3000
Species Reactivity:
Human
Immunogen:
Synthesized peptide derived from N-terminal of human ZNF397.
Form:
Liquid
Storage Buffer:
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
Western blot analysis of extracts from HeLa cells and HUVEC cells, using ZNF397 antibody.
Background:
Isoform 3 acts as a DNA-dependent transcriptional repressor. The MGC Project Team, Genome Res. 14:2121-2127(2004).
Synonyms:
Zinc finger protein 397; Zinc finger and SCAN domain-containing protein 15; Zinc finger protein 47; ZNF397; ZNF47
UniProt Protein Function:
ZNF397: Isoform 3 acts as a DNA-dependent transcriptional repressor. Belongs to the krueppel C2H2-type zinc-finger protein family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:
Protein type:DNA-binding; C2H2-type zinc finger protein; Transcription factor
Chromosomal Location of Human Ortholog: 18q12.2
Cellular Component: cytoplasm; nucleus
Molecular Function:metal ion binding; protein binding; sequence-specific DNA binding; transcription factor activity
Biological Process: regulation of transcription, DNA-dependent; transcription, DNA-dependent
NCBI Summary:
This gene encodes a protein with a N-terminal SCAN domain, and the longer isoform contains nine C2H2-type zinc finger repeats in the C-terminal domain. The protein localizes to centromeres during interphase and early prophase, and different isoforms can repress or activate transcription in transfection studies. Multiple transcript variants encoding different isoforms have been found for this gene. Additional variants have been described, but their biological validity has not been determined. [provided by RefSeq, Oct 2009]
