The ZNF566 Polyclonal Antibody (CAB24543) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high specificity and sensitivity towards ZNF566 in human samples, making it an ideal choice for use in Western blot applications. By binding to the ZNF566 protein, this antibody enables the detection and analysis of ZNF566 expression in various cell types, offering valuable insights into its function in processes such as development, differentiation, and disease pathways.ZNF566, a member of the zinc-finger protein family, is known for its involvement in transcriptional regulation and its impact on cellular function.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
ZNF566 Polyclonal Antibody
SKU:
CAB24543
Size:
20μL, 100μL
Reactivity:
Human
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:
Email for sequence
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:1000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
ZNF566, zinc finger protein 566, 66
Positive Sample:
SH-SY5Y, 293T
Cellular Localization:
Nucleus.
Calculated MW:
49kDa
Observed MW:
56kDa/49kDa
Predicted to enable DNA-binding transcription repressor activity, RNA polymerase II-specific and RNA polymerase II transcription regulatory region sequence-specific DNA binding activity. Predicted to be involved in negative regulation of transcription by RNA polymerase II. Predicted to be located in nucleus. Predicted to be integral component of membrane.
Purification Method
Affinity purification
Gene ID
84924
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from SH-SY5Y cells, using [KO Validated] ZNF566 Rabbit pAb (CAB24543) at 1:800 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Western blot analysis of lysates from wild type (WT) and ZNF566 knockdown (KD) 293T cells using ZNF566 Rabbit pAb (CAB24543) at 1:600 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
![Western blot analysis of SH-SY5Y, using [KO Validated] ZNF566 Rabbit pAb (CAB24543) at 1:800 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.](https://cdn11.bigcommerce.com/s-h68l9z2lnx/images/stencil/608x608/products/224378/583135/A24543_1__84892.1701346950.jpg?c=2 "Western blot analysis of SH-SY5Y, using [KO Validated] ZNF566 Rabbit pAb (CAB24543) at 1:800 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.")
![Western blot analysis of SH-SY5Y, using [KO Validated] ZNF566 Rabbit pAb (CAB24543) at 1:800 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.](https://cdn11.bigcommerce.com/s-h68l9z2lnx/images/stencil/1280x1280/products/224378/583135/A24543_1__84892.1701346950.jpg?c=2&imbypass=on "Western blot analysis of SH-SY5Y, using [KO Validated] ZNF566 Rabbit pAb (CAB24543) at 1:800 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.")
