The 15d-PGJ2 (15-Deoxy-delta12,14-Prostaglandin J2) ELISA Kit is a cutting-edge tool designed for the precise measurement of 15d-PGJ2 levels in various biological samples. With exceptional sensitivity and specificity, this kit delivers accurate and consistent results, making it an invaluable resource for research in a variety of fields.15d-PGJ2 is a biologically active prostaglandin known for its anti-inflammatory and anti-cancer properties. It is involved in regulating cell growth, apoptosis, and immune response, making it a key player in various pathological conditions.
The ability to quantify 15d-PGJ2 levels can provide valuable insights into disease mechanisms and potentially aid in the development of novel therapeutic strategies.Whether studying inflammation, cancer, or metabolic disorders, the 15d-PGJ2 ELISA Kit offers a reliable and efficient platform for detecting and quantifying this important biomolecule. Trust in this advanced assay kit to enhance your research and uncover new possibilities in the fascinating world of biochemistry.
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with 15d-PGJ2. During the reaction, 15d-PGJ2 in the sample or standard competes with a fixed amount of 15d-PGJ2 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to 15d-PGJ2. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of 15d-PGJ2 in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-107
89-100
96-108
Average (%)
100
95
103
1:4
Range (%)
97-113
91-105
90-106
Average (%)
104
98
97
1:8
Range (%)
101-114
88-102
86-98
Average (%)
107
94
92
1:16
Range (%)
95-110
90-103
87-100
Average (%)
101
96
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-106
99
EDTA plasma (n=5)
94-107
99
Cell culture media (n=5)
93-106
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
422
851.5
2917.8
453.9
842.5
3029.8
Standard deviation
28.3
40.0
116.7
26.8
43.0
160.6
C V (%)
6.71
4.7
4.0
5.9
5.1
5.3
Sample type &Sample volume:
Serum, plasma and other biological fluids; 50μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of 15d-PGJ2 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes 15d-PGJ2 in samples. No significant cross-reactivity or interference between 15d-PGJ2 and analogues was observed.
