Description
5-Color Prestained Protein Ladder (11-250 kDa)
The 5-Color Prestained Protein Ladder (11-250 kDa) is a high-performance protein marker designed for convenience and precision in SDS-PAGE and western blotting applications. This ladder features five distinct colors and 11 sharp protein bands that enable accurate protein sizing across a broad 11 to 250 kDa range. Requiring only 3–5 µL per well, it provides efficient usage while maintaining clear band visibility. The direct-to-gel loading formula allows the ladder to be used immediately without additional preparation, helping streamline laboratory workflows and save valuable time.
Key Advantages:
- 5 vibrant colors for easy band identification and gel orientation.
- 11 sharp protein bands for precise molecular weight determination.
- Broad 11–250 kDa range suitable for a wide variety of protein analyses.
- Low loading volume requirement of only 3–5 µL per well.
- Direct-to-gel loading formula for immediate and convenient use.
Designed for modern laboratory needs, this prestained protein ladder helps researchers achieve clear, reliable, and reproducible results while improving efficiency during electrophoresis and western blot workflows.
| Product Name: | 5-Color Prestained Protein Ladder (11-250 kDa) | ||||||||
| SKU: | ABWB0001 | ||||||||
| Number of Bands: | 11 recombinant proteins | ||||||||
| Molecular Weight Range: | 11–250 kDa | ||||||||
| Band Colors: | Blue (majority), Red (80 kDa), Green (25 kDa), Yellow (15 kDa), Purple (250 kDa) | ||||||||
| Usage Recommendation: | 3 μL per lane for 15-well gels, 5 μL per lane for 10-well gels, or 2.5 μL per lane for Western transfer | ||||||||
| Quality Control: | Each batch undergoes strict quality testing. A 5 μL sample of PMX11 resolves 10 visible bands on 4–20% SDS-PAGE (Tris-glycine buffer) and produces distinct reference bands after Western blot transfer to PVDF membranes. | ||||||||
| Storage Buffer Composition: | Each protein band (0.2–0.4 mg/mL) is stored in a stabilizing buffer containing:
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The 5-Color Prestained Protein Ladder (11-250 kDa) is a combination of 11 pre-stained proteins with molecular weights from 11 to 250 kDa. The 11 recombinant proteins are covalently coupled with blue chromophore, while a red band at 80 kDa, a green band at 25 kDa, a newly designed yellow band at 15 kDa, and a purple band at 250 kDa serve as reference bands. The 5-Color Prestained Protein Ladder (11-250 kDa) keeps track of the size and separation of proteins during SDS-polyacrylamide gel electrophoresis, approximating the target protein size and validating the Western transfer efficiency on PVDF, nylon, or nitrocellulose membranes.
Refer to the 5-Color Prestained Protein Ladder (11-250 kDa) migration pattern under various electrophoresis conditions.
Load 3 μL or 5 μL per lane for optimal visualization on 15-well or 10-well mini-gels, respectively, or 2.5 μL per lane for standard Western transfers.
Note: Molecular weights were determined relative to an unstained protein ladder under each electrophoresis condition. For precise protein sizing, compare with additional calibration data.
Materials Required but not provided
- Vertical Electrophoresis system
- Power supplies
- Vortex or equivalent
- Microcentrifuge
Figure 1. This Assay Genie marker was evaluated using an accelerated stability test by storing samples at 25°C for a designated period.
The conversion is as follows:
- Accelerated 9 days ≈ −20°C for 204 days (0.6 years)
- Accelerated 17 days ≈ −20°C for 385 days (1.1 years)
- Accelerated 34 days ≈ −20°C for 769 days (2.1 years)
- Accelerated 41 days ≈ −20°C for 928 days (2.5 years)
Thermo-Novex 4–20% TG gel was used to run SDS-PAGE in Tris-glycine buffer. 5 μL of protein ladder sample was loaded into each lane.
