The 6-Keto PGF1a (6-Keto Prostaglandin F1a) ELISA Kit is a reliable and accurate tool for detecting levels of 6-Keto PGF1a in various biological samples. This kit offers high sensitivity and specificity, ensuring consistent and reproducible results for a wide range of research applications.6-Keto PGF1a is a metabolite of prostacyclin, a key player in the regulation of inflammation and vascular tone.
It is known to have important implications in conditions such as cardiovascular diseases, thrombosis, and inflammatory disorders. Monitoring 6-Keto PGF1a levels can provide valuable insights into these physiological and pathological processes, making this kit essential for researchers studying these areas and developing potential therapeutic interventions.
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with 6-keto-PGF1a. During the reaction, 6-keto-PGF1a in the sample or standard competes with a fixed amount of 6-keto-PGF1a on the solid phase supporter for sites on the Biotinylated Detection Ab specific to 6-keto-PGF1a. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of 6-keto-PGF1a in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-106
97-111
89-103
Average (%)
98
103
96
1:4
Range (%)
89-103
90-104
95-108
Average (%)
95
96
101
1:8
Range (%)
88-102
88-102
98-116
Average (%)
94
93
106
1:16
Range (%)
91-105
92-109
100-116
Average (%)
96
100
106
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-104
96
EDTA plasma (n=5)
83-97
90
Cell culture media (n=5)
87-103
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
54.4
139.6
366.2
50.5
151.2
402.5
Standard deviation
3.6
5.7
17.9
3.0
8.0
14.9
C V (%)
6.62
4.08
4.89
5.94
5.29
3.7
Sample type &Sample volume:
serum, plasma and other biological fluids; 50μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of 6-keto-PGF1a concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes 6-keto-PGF1a in samples. No significant cross-reactivity or interference between 6-keto-PGF1a and analogues was observed.
