The ABLIM1 Polyclonal Antibody (PAC047274) is specifically designed for researchers studying ABLIM1, a protein involved in actin-binding and cytoskeleton organization. This antibody, raised in rabbits, is highly reactive with human samples and has been validated for use in Western blot applications. By binding to the ABLIM1 protein, this antibody allows for detection and analysis in various cell types, making it an ideal tool for research in cell biology and cancer biology.ABLiM1, also known as actin-binding LIM protein 1, plays a crucial role in mediating actin dynamics and cytoskeletal rearrangements, which are essential processes for cell migration, adhesion, and signaling.
Its involvement in these fundamental cellular functions makes ABLIM1 a key target for investigation in cancer metastasis, cell motility, and cytoskeletal regulation. Understanding the function and regulation of ABLIM1 is essential for unraveling the mechanisms underlying these processes and identifying potential therapeutic targets for cancer and other diseases.
Western Blot. Positive WB detected in: Hela whole cell lysate, HEK293 whole cell lysate, Mouse heart tissue. All lanes: ABLIM1 antibody at 3.4µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 88, 82, 49, 47, 53, 85 kDa. Observed band size: 88 kDa..
Immunofluorescent analysis of HepG2 cells using PACO47274 at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunohistochemistry of paraffin-embedded human heart tissue using PACO47274 at dilution of 1:100.
Background:
May act as scaffold protein (By similarity). May play a role in the development of the retina. Has been suggested to play a role in axon guidance.
Synonyms:
Actin-binding LIM protein 1 (abLIM-1) (Actin-binding LIM protein family member 1) (Actin-binding double zinc finger protein) (LIMAB1) (Limatin), ABLIM1, ABLIM KIAA0059 LIMAB1
UniProt Protein Function:
abLIM: a cytoskeletal LIM protein consisting of a C-terminal cytoskeletal domain fused to an N-terminal domain of four double zinc finger motifs. The C-terminal domain is 50% identical to dematin, an actin-bundling protein of the erythroid cytoskeleton. Undergoes extensive phosphorylation in light-adapted retinas in vivo and its developmental expression in the retina coincides with the elaboration of photoreceptor inner and outer segments. Three distinct splice variant isoforms have been described: ABLIM-l, ABLIM-m, ABLIM-s.Protein type: Cytoskeletal; Motility/polarity/chemotaxis; Actin-bindingChromosomal Location of Human Ortholog: 10q25Cellular Component: lamellipodium; cytoplasm; stress fiber; actin cytoskeletonMolecular Function: protein binding; zinc ion binding; actin bindingBiological Process: lamellipodium biogenesis; axon guidance; organ morphogenesis; visual perception; cilium biogenesis; positive regulation of transcription from RNA polymerase II promoter; cytoskeleton organization and biogenesis
UniProt Protein Details:
NCBI Summary:
This gene encodes a cytoskeletal LIM protein that binds to actin filaments via a domain that is homologous to erythrocyte dematin. LIM domains, found in over 60 proteins, play key roles in the regulation of developmental pathways. LIM domains also function as protein-binding interfaces, mediating specific protein-protein interactions. The protein encoded by this gene could mediate such interactions between actin filaments and cytoplasmic targets. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]
