Description
ACE1 Antibody (CAB2805)
The ACE1 Antibody (CAB2805) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody is produced in rabbits and has been validated for use in Western blot applications with high reactivity towards human samples.ACE, also known as angiotensin-converting enzyme, is a critical player in the control of blood pressure and is a target for medications used to treat hypertension and heart failure.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
| Product Name: | ACE1 Antibody |
| SKU: | CAB2805 |
| Size: | 20μL, 100μL |
| Reactivity: | Human, Mouse, Rat |
| Conjugate: | Unconjugated |
| Immunogen: | Recombinant protein (or fragment).This information is considered to be commercially sensitive. | ||||||
| Sequence: | LHQC DIYR STKA GAKL RKVL QAGS SRPW QEVL KDMV GLDA LDAQ PLLK YFQP VTQW LQEQ NQQN GEVL GWPE YQWH PPLP DNYP EGID LVTD EAEA SKFV EEYD RTSQ VVWN EYAE ANWN YNTN ITTE TSKI LLQK NMQI ANHT LKYG TQAR KFDV NQLQ NTTI KRII KKVQ DLER AALP AQEL EEYN KILL DMET TYSV ATVC H | ||||||
| Tested Applications: | WB IHC-P ELISA | ||||||
| Recommended Dilution: |
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| Synonyms: | DCP, ACE1, DCP1, CD143 |
| Positive Sample: | Mouse lung, Mouse kidney, Rat lung |
| Cellular Localization: | Cell Membrane, Cytoplasm, Secreted, Single-Pass Type I Membrane Protein. |
| Calculated MW: | 150kDa |
| Observed MW: | 170kDa |
This gene encodes an enzyme involved in blood pressure regulation and electrolyte balance. It catalyzes the conversion of angiotensin I into a physiologically active peptide angiotensin II. Angiotensin II is a potent vasopressor and aldosterone-stimulating peptide that controls blood pressure and fluid-electrolyte balance. This angiotensin converting enzyme (ACE) also inactivates the vasodilator protein, bradykinin. Accordingly, the encoded enzyme increases blood pressure and is a drug target of ACE inhibitors, which are often prescribed to reduce blood pressure. This enzyme additionally plays a role in fertility through its ability to cleave and release GPI-anchored membrane proteins in spermatozoa. Many studies have associated the presence or absence of a 287 bp Alu repeat element in this gene with the levels of circulating enzyme. This polymorphism, as well as mutations in this gene, have been implicated in a wide variety of diseases including cardiovascular pathophysiologies, psoriasis, renal disease, stroke, and Alzheimer's disease. Regulation of the homologous ACE2 gene may be involved in progression of disease caused by several human coronaviruses, including SARS-CoV and SARS-CoV-2. Alternative splicing results in multiple transcript variants encoding both somatic (sACE) and male-specific testicular (tACE) isoforms.
| Purification Method | Affinity purification |
| Gene ID | 1636 |
| RRID | AB_2764643 |
| Buffer Information | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3. |
 | Western blot analysis of various lysates using ACE1 Rabbit pAb (CAB2805) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s. |
 | Immunohistochemistry analysis of paraffin-embedded Rat lung using ACE11 Rabbit pAb (CAB2805) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining. |
