The Acetyl-Histone H2B (Lys20) Polyclonal Antibody (PACO00161) is a valuable tool for researchers studying histone modifications and their impact on gene expression regulation. This antibody, generated in rabbits, demonstrates high reactivity with human samples and has been validated for use in various applications, including Western blotting.Histone H2B lysine 20 acetylation is an important post-translational modification that plays a role in regulating chromatin structure and gene transcription. By specifically targeting this modification, researchers can gain insights into the epigenetic mechanisms that govern cellular processes such as DNA replication, repair, and apoptosis.
The Acetyl-Histone H2B (Lys20) Polyclonal Antibody enables precise detection and analysis of acetylated histone H2B in a variety of cell types, making it a versatile tool for studies in epigenetics, cancer biology, and developmental biology. Understanding the impact of histone modifications like acetylation on gene regulation is crucial for deciphering the complex mechanisms that drive normal and pathological cellular processes.
Antibody Name:
Acetyl-Histone H2B (Lys20) Antibody
Antibody SKU:
PACO00161
Size:
50ug
Host Species:
Rabbit
Tested Applications:
WB
Recommended Dilutions:
Species Reactivity:
Human, Mouse, Rat
Immunogen:
synthetic Peptide
Form:
Liquid
Storage Buffer:
PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
H2B1A: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Transcribed exclusively in testis, and the corresponding protein is also present in mature sperm. Belongs to the histone H2B family.Protein type: DNA-bindingChromosomal Location of Human Ortholog: 6p22.2Cellular Component: extrinsic to plasma membrane; nuclear chromosome, telomeric region; nucleoplasm; nucleosome; nucleusMolecular Function: DNA binding; protein heterodimerization activityBiological Process: establishment and/or maintenance of chromatin architecture; inflammatory response; nucleosome assembly; nucleosome disassembly; plasminogen activation; positive regulation of binding; spermatogenesis, exchange of chromosomal proteins
UniProt Protein Details:
NCBI Summary:
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a testis/sperm-specific member of the histone H2B family. Transcripts from this gene contain a palindromic termination element. [provided by RefSeq, Aug 2015]
