The Acetyl-Histone H3-K9/K14/K18/K23/K27 Antibody (CAB17917) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, specifically targets acetylated forms of Histone H3 at multiple lysine residues, allowing for the detection and analysis of histone modifications involved in gene expression regulation.Histone H3 acetylation at these sites is known to be associated with active transcription and chromatin accessibility, making this antibody an essential reagent for studies in gene regulation, cell differentiation, and development. Validated for use in applications such as ChIP, Western blot, and immunofluorescence, this antibody is highly reactive with human samples, providing reliable and consistent results for researchers working in the field of molecular biology.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat, Other (Wide Range Predicted) samples.
Product Name:
Acetyl-Histone H3-K9/K14/K18/K23/K27 Antibody
SKU:
CAB17917
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat, Other (Wide Range Predicted)
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
KSTG GKAP RKQL ATKA ARK
Tested Applications:
WBIF/ICCELISA
Recommended Dilution:
WB
1:1000 - 1:5000
IF/ICC
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3.
Purification Method
Affinity purification
Gene ID
8290 8350
RRID
AB_2768210
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from HeLa cells, using Acetyl-Histone H3-K9/K14/K18/K23/K27 Rabbit pAb (CAB17917) at 1:5000 dilution. HeLa cells were treated with TSA (1 uM) at 37℃ for 18 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Immunofluorescence analysis of HeLa treated with TSA(1 uM,4 hours) and HeLa cells using Acetyl-Histone H3-K9/K14/K18/K23/K27 Rabbit pAb(CAB17917) at a dilution of 1:100 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L)(CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
