Acetyl-Histone H3-K9/K14/K18/K23/K27 Rabbit Polyclonal Antibody (CAB17917)

(No reviews yet) Write a Review
SKU:
CAB17917
$119 - $383

Description

Technical Manual

Acetyl-Histone H3-K9/K14/K18/K23/K27 Rabbit Polyclonal Antibody

Product Name:Acetyl-Histone H3-K9/K14/K18/K23/K27 Rabbit Polyclonal Antibody
Product SKU:CAB17917
Size:20uL, 50 uL
Host Species:Rabbit
Purification Method:Affinity purification
Isotype:IgG
Background:

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.

Immunogen:A synthetic peptide of human Acetyl-Histone H3-K9/K14/K18/K23/K27.
Sequence:

Email for sequence

Gene ID:8350
Swiss Prot:P68431
Synonyms:H3/A; H3C2; H3C3; H3C4; H3C6; H3C7; H3C8; H3FA; H3C10; H3C11; H3C12; HIST1H3A
Calculated MW:15kDa
Observed MW:17kDa
Reactivity:Human, Mouse, Rat, Other (Wide Range)
Application:WB IHC IF IP ChIP
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 ChIP 1:20 - 1:100
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting Immunohistochemistry Immunofluorescence 
Positive Samples:HeLa, NIH/3T3, C6
Cellular Location:Chromosome, Nucleus

Product Images

Acetyl-Histone H3-K9/K14/K18/K23/K27 Rabbit Polyclonal Antibody (CAB17917)

Western blot - Acetyl-Histone H3-K9/K14/K18/K23/K27 Rabbit pAb (CAB17917)

Uniprot Information

UniProt Protein Function:H3: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Belongs to the histone H3 family.
UniProt Protein Details:

Protein type:DNA-binding

Chromosomal Location of Human Ortholog: 6p22.2

Cellular Component: extracellular region; membrane; nuclear chromosome; nuclear chromosome, telomeric region; nucleoplasm; nucleosome; nucleus; protein complex

Molecular Function:cadherin binding; histone binding; protein binding

Biological Process: blood coagulation; cellular protein metabolic process; chromatin silencing at rDNA; DNA replication-dependent nucleosome assembly; establishment and/or maintenance of chromatin architecture; negative regulation of gene expression, epigenetic; nucleosome assembly; positive regulation of gene expression, epigenetic; protein heterotetramerization; RNA-mediated gene silencing; telomere organization and biogenesis

NCBI Summary:Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the small histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015]
UniProt Code:P68431
NCBI GenInfo Identifier:55977055
NCBI Gene ID:8357
NCBI Accession:P68431.2
UniProt Secondary Accession:P68431,P02295, P02296, P16106, Q6ISV8, Q6NWP8, Q6NWP9 Q6NXU4, Q71DJ3, Q93081, A0PJT7, A5PLR1,
UniProt Related Accession:P68431
Molecular Weight:15kDa
NCBI Full Name:Histone H3.1
NCBI Synonym Full Names:histone cluster 1 H3 family member h
NCBI Official Symbol:HIST1H3H 
NCBI Official Synonym Symbols:H3/k; H3FK; H3F1K 
NCBI Protein Information:histone H3.1
UniProt Protein Name:Histone H3.1
UniProt Synonym Protein Names:Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; Histone H3/l
UniProt Gene Name:HIST1H3A 
View AllClose

Additional Information

Product type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Reactivity:
Other (Wide Range)
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
View AllClose

0 Reviews

View AllClose