The ACh (Acetylcholine) ELISA Kit from Assay Genie is specifically designed for the accurate detection of acetylcholine levels in various biological samples, including serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides reliable and reproducible results, making it an ideal tool for researchers in a wide range of fields.Acetylcholine is a neurotransmitter that plays a crucial role in various physiological processes, including muscle contractions, memory, and learning.
Dysregulation of acetylcholine levels has been associated with various neurological disorders, making it a valuable biomarker for studying these conditions.Whether you are studying the nervous system, neuropsychiatric disorders, or muscle physiology, the ACh ELISA Kit from Assay Genie provides a reliable and efficient platform for accurate measurement of acetylcholine levels. Order yours today and take your research to the next level.
Product Name:
ACH (Acetylcholine) ELISA Kit
SKU:
UNES00050
Target:
ACH (Acetylcholine) ELISA Kit (UNES00050)
Size:
96T
Assay type:
Competitive-ELISA
Assay time:
2.0h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with ACH. During the reaction, ACH in the sample or standard competes with a fixed amount of ACH on the solid phase supporter for sites on the Biotinylated Detection Ab specific to ACH. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of ACH in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-108
93-106
88-101
Average (%)
100
98
94
1:4
Range (%)
97-112
89-101
89-101
Average (%)
105
95
96
1:8
Range (%)
99-109
88-102
91-104
Average (%)
104
95
97
1:16
Range (%)
98-112
94-104
92-107
Average (%)
105
99
98
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-102
96
EDTA plasma (n=5)
88-102
93
Cell culture media (n=5)
87-102
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
50.9
124.3
438.1
55.3
116.5
429.8
Standard deviation
2.6
6.1
13.6
3.5
5.7
15.5
C V (%)
5.11
4.91
3.1
6.33
4.89
3.61
Sample type &Sample volume:
serum, plasma and other biological fluids; 50μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of ACH concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes ACH in samples. No significant cross-reactivity or interference between ACH and analogues was observed.
