Description
system_update_altDatasheet
| Product Name: | Acid Phosphatase Activity Assay Kit |
| Product Code: | MAES0082 |
| Product Size: | 96 Assays |
| Sample Type: | Serum, plasma, hydrothorax, urine, cells, cell culture supernatant, animal tissue |
| Assay Time: | 45 min |
| Instrument: | Microplate reader |
| Sensitivity: | 1.40 U/100 mL |
| Detection Range: | 1.40-40 U/100 mL |
| Recovery Rate: | 104 |
| Inter CV: | 9.4 |
| Inter CV: | 2.1 |
Acid phosphatase (ACP) is a kind of enzyme which catalyzes the hydrolysis of phosphate monoester to phosphoric acid under acidic conditions. There are different acid phosphatase isozymes in different organs. These isozymes differ greatly in tissue and chromosome origin, molecular weight, amino acid homology, sequence length, and resistance to L-tartrate or fluoride.
Acid phosphatase decomposes disodium phenyl phosphate under acidic conditions to produce free phenol and phosphoric acid. Phenol acts with 4-aminoantipyrine in alkaline solution, and oxidizes to a derivative of red quinone by potassium ferricyanide. The activity of the ACP can be calculated by measuring the OD value at 520 nm.
| Shelf Life: | 3 Months |
| Storage: | 2-8°C |
| Shipping: | Gel Pack |
| Research Area: | Enzymes, Others |
