The ACSS2 Antibody (CAB6472) is a high-quality antibody developed for reliable detection and analysis of target proteins. This gene encodes a cytosolic enzyme that catalyzes the activation of acetate for use in lipid synthesis and energy generation. The protein acts as a monomer and produces acetyl-CoA from acetate in a reaction that requires ATP. Expression of this gene is regulated by sterol regulatory element-binding proteins, transcription factors that activate genes required for the synthesis of cholesterol and unsaturated fatty acids. Alternative splicing results in multiple transcript variants. RRID AB_2767073 Gene ID 55902 Swiss Prot Synonym ACS; ACSA; ACAS2; ACECS; AceCS1; dJ1161H23.1; ACSS2
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
ACSS2 Antibody
SKU:
CAB6472
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Clone Number:
-
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBIF/ICCELISA
Recommended Dilution:
WB
1:500 - 1:2000
IF
/
ICC
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
HepG2, COS-7, U-87MG, C2C12, Mouse liver, Mouse brain, Rat liver, Rat kidney
Cellular Localization:
Cytoplasm.
Calculated MW:
79kDa
Observed MW:
78kDa
This gene encodes a cytosolic enzyme that catalyzes the activation of acetate for use in lipid synthesis and energy generation. The protein acts as a monomer and produces acetyl-CoA from acetate in a reaction that requires ATP. Expression of this gene is regulated by sterol regulatory element-binding proteins, transcription factors that activate genes required for the synthesis of cholesterol and unsaturated fatty acids. Alternative splicing results in multiple transcript variants. RRID AB_2767073 Gene ID 55902 Swiss Prot Synonym ACS; ACSA; ACAS2; ACECS; AceCS1; dJ1161H23.1; ACSS2
Purification Method:
Affinity purification
Gene ID:
55902
RRID:
AB_2767073
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using ACSS2 Rabbit pAb (CAB6472) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 5s.
Confocal immunofluorescence analysis of NIH-3T3 cells using ACSS2 Rabbit pAb (CAB6472) at dilution of 1:200. Blue: DAPI for nuclear staining.
