The Actin (Plant Specific) Monoclonal Antibody (CAB23959) is a high-quality antibody developed for reliable detection and analysis of target proteins. Actin is an ancient conserved protein that is encoded by multiple isovariants in multi-cellular organisms. There are eight functional actin genes in the Arabidopsis genome.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Widely Range, Oryza sativa, Arabidopsis thaliana samples.
Product Name:
Actin (Plant Specific) Monoclonal Antibody
SKU:
CAB23959
Size:
100μL
Reactivity:
Widely Range, Oryza sativa, Arabidopsis thaliana
Clone Number:
ARC62861
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:10000 - 1:60000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
ACT12, Actin(Plant Specific)
Positive Sample:
Oryza sativa, Arabidopsis thaliana
Cellular Localization:
Cytoplasm, Cytoskeleton.
Calculated MW:
45kDa
Observed MW:
45kDa
Actin is an ancient conserved protein that is encoded by multiple isovariants in multi-cellular organisms. There are eight functional actin genes in the Arabidopsis genome.
Purification Method
Affinity purification
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Oryza sativa, using Actin(Plant Specific) Rabbit mAb (CAB23959) at 1:10000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Western blot analysis of Arabidopsis thaliana, using Actin(Plant Specific) Rabbit mAb (CAB23959) at 1:10000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
