The β-Actin Rabbit pAb (CABC006) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, shows high reactivity with actin in human samples and is validated for use in Western blot applications. By binding specifically to actin, this antibody allows for the detection and analysis of actin expression in various cell types.Actin is essential for numerous cellular processes, including cell division, muscle contraction, and cell migration. Dysregulation of actin dynamics has been implicated in various diseases, such as cancer and neurological disorders.
This antibody is validated for use in WB applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
β-Actin Rabbit pAb
SKU:
CABC006
Size:
100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, integrity, and intercellular signaling. The encoded protein is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins that are ubiquitously expressed. Mutations in this gene cause Baraitser-Winter syndrome 1, which is characterized by intellectual disability with a distinctive facial appearance in human patients. Numerous pseudogenes of this gene have been identified throughout the human genome.
Purification Method
Affinity purification
Gene ID
60
RRID
AB_2768236
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using β-Actin Rabbit pAb (CABC006) at 1:125000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30 s.
