Activator protein 2 (AP-2/TFAP2A) Polyclonal Antibody (CAB21727)
The Activator protein 2 (AP-2/TFAP2A) Polyclonal Antibody (CAB21727) is a high-quality antibody developed for reliable detection and analysis of target proteins. The protein encoded by this gene is a transcription factor that binds the consensus sequence 5'-GCCNNNGGC-3'. The encoded protein functions as either a homodimer or as a heterodimer with similar family members. This protein activates the transcription of some genes while inhibiting the transcription of others. Defects in this gene are a cause of branchiooculofacial syndrome (BOFS). Three transcript variants encoding different isoforms have been found for this gene.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Rat samples.
Product Name:
Activator protein 2 (AP-2/TFAP2A) Polyclonal Antibody
SKU:
CAB21727
Size:
100μL, 20μL
Reactivity:
Human, Rat
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:1000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
AP-2, BOFS, AP2TF, TFAP2, AP-2alpha, Activator protein 2 (AP-2/TFAP2A)
Positive Sample:
HeLa, C6
Cellular Localization:
Nucleus.
Calculated MW:
48kDa
Observed MW:
50kDa
The protein encoded by this gene is a transcription factor that binds the consensus sequence 5'-GCCNNNGGC-3'. The encoded protein functions as either a homodimer or as a heterodimer with similar family members. This protein activates the transcription of some genes while inhibiting the transcription of others. Defects in this gene are a cause of branchiooculofacial syndrome (BOFS). Three transcript variants encoding different isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
7020
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Activator protein 2 (AP-2/TFAP2A) Rabbit pAb (CAB21727) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
