The ACTN3 Antibody (CAB12797) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, displays strong reactivity towards human samples and has been thoroughly validated for use in Western blot applications. By specifically binding to the ACTN3 protein, this antibody enables accurate detection and analysis in a variety of cell types, making it a valuable asset for investigations in the fields of exercise physiology, sports science, and muscle biology.ACTN3 is known for its role in muscle contraction, particularly in activities requiring explosive power such as sprinting and weightlifting.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
ACTN3 Antibody
SKU:
CAB12797
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
ACTN3D, ACTN3
Positive Sample:
LO2, SP2/0
Cellular Localization:
Cortical Actin Cytoskeleton, Cytosol, Extracellular Exosome, Plasma Membrane, Z Disc.
Calculated MW:
103kDa
Observed MW:
103kDa
This gene encodes a member of the alpha-actin binding protein gene family. The encoded protein is primarily expressed in skeletal muscle and functions as a structural component of sarcomeric Z line. This protein is involved in crosslinking actin containing thin filaments. An allelic polymorphism in this gene results in both coding and non-coding variants; the reference genome represents the coding allele. The non-functional allele of this gene is associated with elite athlete status.
Purification Method
Affinity purification
Gene ID
89
RRID
AB_2759636
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using ACTN3 Rabbit pAb (CAB12797) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Immunohistochemistry analysis of paraffin-embedded Mouse skeletal muscle using ACTN3 Rabbit pAb (CAB12797) at dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
