The Adenosine Deaminase (ADA) Monoclonal Antibody (CAB5151) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, developed using innovative rabbit monoclonal technology, demonstrates high specificity and sensitivity towards human ADA samples, making it a reliable choice for various research applications.ADA, also known as adenosine deaminase, plays a crucial role in maintaining purine balance and nucleic acid metabolism in the body. Dysregulation of ADA activity has been linked to various diseases, including immunodeficiency disorders and certain types of cancer. By targeting ADA with this monoclonal antibody, researchers can study the enzyme's expression levels and localization in different cell types, providing valuable insights into its functional significance.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Adenosine Deaminase (ADA) Monoclonal Antibody
SKU:
CAB5151
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC1152
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
This gene encodes an enzyme that catalyzes the hydrolysis of adenosine to inosine in the purine catabolic pathway. Various mutations have been described for this gene and have been linked to human diseases related to impaired immune function such as severe combined immunodeficiency disease (SCID) which is the result of a deficiency in the ADA enzyme. In ADA-deficient individuals there is a marked depletion of T, B, and NK lymphocytes, and consequently, a lack of both humoral and cellular immunity. Conversely, elevated levels of this enzyme are associated with congenital hemolytic anemia.
Purification Method
Affinity purification
Gene ID
100
RRID
AB_2863468
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using Adenosine Deaminase (ADA) Rabbit mAb (CAB5151) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
Western blot analysis of lysates from Mouse stomach, using Adenosine Deaminase (ADA) Rabbit mAb (CAB5151) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 45s.
