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[KO Validated] Adenosine Deaminase (ADA) Rabbit Polyclonal Antibody (CAB18029)

SKU:
CAB18029
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology
€139 - €419
Frequently bought together:

Description

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Product Name:[KO Validated] Adenosine Deaminase (ADA) Rabbit Polyclonal Antibody
SKU:CAB18029
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-363 of human Adenosine Deaminase (Adenosine Deaminase (ADA)) (NP_000013.2).
Sequence:MAQT PAFD KPKV ELHV HLDG SIKP ETIL YYGR RRGI ALPA NTAE GLLN VIGM DKPL TLPD FLAK FDYY MPAI AGCR EAIK RIAY EFVE MKAK EGVV YVEV RYSP HLLA NSKV EPIP WNQA EGDL TPDE VVAL VGQG LQEG ERDF GVKA RSIL CCMR HQPN WSPK VVEL CKKY QQQT VVAI DLAG DETI PGSS LLPG HVQA YQEA VKSG IHRT VHAG EVGS AEVV KEAV DILK TERL GHGY HTLE DQAL YNRL RQEN MHFE ICPW SSYL TGAW KPDT EHAV IRLK NDQA NYSL NTDD PLIF KSTL DTDY QMTK RDMG FTEE EFKR LNIN AAKS SFLP EDEK RELL DLLY KAYG MPPS ASAG QNL
Tested Applications:WB ELISA
Recommended Dilution:WB,1:500 - 1:2000
Synonyms:ADA1; A)
Positive Sample:HeLa
Conjugate:Unconjugated
Cellular Localization:Cell junction, Cell membrane, Cytoplasm, Cytoplasmic vesicle lumen, Extracellular side, Peripheral membrane protein.
Calculated MW:41kDa
Observed MW:41kDa

This gene encodes an enzyme that catalyzes the hydrolysis of adenosine to inosine in the purine catabolic pathway. Various mutations have been described for this gene and have been linked to human diseases related to impaired immune function such as severe combined immunodeficiency disease (SCID) which is the result of a deficiency in the ADA enzyme. In ADA-deficient individuals there is a marked depletion of T, B, and NK lymphocytes, and consequently, a lack of both humoral and cellular immunity. Conversely, elevated levels of this enzyme are associated with congenital hemolytic anemia.

Purification Method:Affinity purification
Gene ID:100
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of lysates from wild type (WT) and Adenosine Deaminase (Adenosine Deaminase (ADA)) knockout (KO) HeLa cells, using [KO Validated] Adenosine Deaminase (ADA) Rabbit pAb (CAB18029) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5s.

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