The ADAM5 Polyclonal Antibody (CAB6196) is a high-quality antibody developed for reliable detection and analysis of target proteins. Predicted to be involved in binding activity of sperm to zona pellucida and cell adhesion. RRID AB_2766807 Gene ID 255926 Swiss Prot Synonym ADAM5P; TMDCII; ADAM5
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
ADAM5 Polyclonal Antibody
SKU:
CAB6196
Size:
100μL, 20μL
Reactivity:
Human, Mouse
Clone Number:
-
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:100 - 1:500
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
ADAM5P, TMDCII, ADAM5
Positive Sample:
Mouse testis, Mouse ovary
Cellular Localization:
-
Calculated MW:
47kDa
Observed MW:
50kDa
Predicted to be involved in binding activity of sperm to zona pellucida and cell adhesion. RRID AB_2766807 Gene ID 255926 Swiss Prot Synonym ADAM5P; TMDCII; ADAM5
Purification Method:
Affinity purification
Gene ID:
255926
RRID:
AB_2766807
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using ADAM5 Rabbit pAb (CAB6196) at 1:400 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 180s.
