The ADAM5 Polyclonal Antibody (CAB6196) is a high-quality antibody developed for reliable detection and analysis of target proteins. Predicted to be involved in binding activity of sperm to zona pellucida and cell adhesion.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
ADAM5 Polyclonal Antibody
SKU:
CAB6196
Size:
100μL, 20μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:100 - 1:500
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
ADAM5P, TMDCII, ADAM5
Positive Sample:
Mouse testis, Mouse ovary
Calculated MW:
47kDa
Observed MW:
50kDa
Predicted to be involved in binding activity of sperm to zona pellucida and cell adhesion.
Purification Method
Affinity purification
Gene ID
255926
RRID
AB_2766807
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using ADAM5 Rabbit pAb (CAB6196) at 1:400 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 180s.
