The ADAMTS13 Antibody (CAB8482) is a high-quality antibody developed for reliable detection and analysis of target proteins. The antibody, raised in rabbits, is highly reactive with human samples and is validated for use in various applications such as Western blot and immunohistochemistry.ADAMTS13 plays a crucial role in preventing blood clots by regulating the size of von Willebrand factor. Dysregulation of ADAMTS13 activity has been associated with thrombotic disorders such as thrombotic thrombocytopenic purpura (TTP).
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
ADAMTS13 Antibody
SKU:
CAB8482
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
This gene encodes a member of a family of proteins containing several distinct regions, including a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. The enzyme encoded by this gene specifically cleaves von Willebrand Factor (vWF). Defects in this gene are associated with thrombotic thrombocytopenic purpura. Alternative splicing results in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
11093
RRID
AB_2768247
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using ADAMTS13 Rabbit pAb (CAB8482) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 5s.
Immunofluorescence analysis of NIH-3T3 cells using ADAMTS13 Rabbit pAb (CAB8482) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
