The ADH1B Antibody (CAB1431) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly reactive with human samples and has been validated for use in Western blot applications. By binding to the ADH1B protein, this antibody enables precise detection and analysis in a variety of cell types, making it an essential tool for studies in alcohol metabolism, liver function, and related research areas.ADH1B, a member of the alcohol dehydrogenase family, plays a crucial role in the breakdown of alcohol in the liver. Variations in the ADH1B gene have been linked to differences in alcohol metabolism and susceptibility to alcohol-related diseases.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Rat samples.
Product Name:
ADH1B Antibody
SKU:
CAB1431
Size:
20μL, 100μL
Reactivity:
Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
ADH2, HEL-S-117, ADH1B
Positive Sample:
Rat liver
Cellular Localization:
Cytoplasm.
Calculated MW:
40kDa
Observed MW:
40kDa
The protein encoded by this gene is a member of the alcohol dehydrogenase family. Members of this enzyme family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. This encoded protein, consisting of several homo- and heterodimers of alpha, beta, and gamma subunits, exhibits high activity for ethanol oxidation and plays a major role in ethanol catabolism. Three genes encoding alpha, beta and gamma subunits are tandemly organized in a genomic segment as a gene cluster. Two transcript variants encoding different isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
125
RRID
AB_2761173
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from rat liver, using ADH1B Rabbit pAb (CAB1431) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
