The AFG3L2 Polyclonal Antibody (CAB23598) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific and reactive with human samples, making it suitable for use in Western blot applications. By targeting the AFG3L2 protein, this antibody enables precise detection and analysis in various cell types, aiding in investigations related to mitochondrial biology, neurodegenerative diseases, and protein degradation pathways.AFG3L2 is a conserved mitochondrial ATP-dependent metalloprotease, essential for maintaining mitochondrial homeostasis and cellular function.
This antibody is validated for use in WB, IHC-P, IP, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
AFG3L2 Polyclonal Antibody
SKU:
CAB23598
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
This gene encodes a protein localized in mitochondria and closely related to paraplegin. The paraplegin gene is responsible for an autosomal recessive form of hereditary spastic paraplegia. This gene is a candidate gene for other hereditary spastic paraplegias or neurodegenerative disorders.
Purification Method
Affinity purification
Gene ID
10939
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using AFG3L2 Rabbit pAb (CAB23598) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using AFG3L2 Rabbit pAb (CAB23598) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
