Agarose beads-conjugated anti-DDDDK tag VHH Single Domain antibody (CABE125)
Based on the information from the URL provided, the Agarose Beads Conjugated Anti-DDDDK Tag VHH Single Domain Antibody (CABE125) is a powerful tool for researchers studying proteins tagged with the DDDDK motif. This antibody is suitable for use in immunoprecipitation and protein purification experiments, as well as for immunofluorescence and flow cytometry applications.The antibody is conjugated to agarose beads, allowing for efficient capture and isolation of target proteins in complex mixtures. The DDDDK tag, also known as the FLAG tag, is commonly used in protein expression studies for easy detection and purification.
The VHH single domain structure of the antibody ensures high specificity and affinity for the DDDDK tag, enabling precise and reliable results in various experimental setups.By utilizing the Agarose Beads Conjugated Anti-DDDDK Tag VHH Single Domain Antibody (CABE125), researchers can streamline their protein analysis workflows and enhance the sensitivity and efficiency of their experiments. Whether investigating protein-protein interactions, characterizing protein expression levels, or isolating specific protein targets, this antibody offers a versatile and reliable solution for a wide range of research applications.
Product Name:
Agarose beads-conjugated anti-DDDDK tag VHH Single Domain antibody
SKU:
CABE125
Size:
100uL
Isotype:
VHH
Host Species:
Alpaca
Immunogen:
A synthetic peptide corresponding to DDDDK tag.
Tested Applications:
IP
Recommended Dilution:
IP,30μl-50μl Agarose Beads for 100μg-300μg extracts of whole cells
Synonyms:
DDDDK;DDDDK tag;DDDDK-tag
Positive Sample:
293T-GSK3B-Flag
Conjugate:
Agarose Beads
Observed MW:
48kDa/35kDa
FLAG-tag, or FLAG octapeptide, or FLAG epitope, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology, having the sequence motif DYKDDDDK. It has been used for studying proteins in living cells and for protein purification by affinity chromatography. It has been used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits, because its mild purification procedure tends not to disrupt such complexes. It has been used to obtain proteins of sufficient purity and quality to carry out 3D structure determination by x-ray crystallography.A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against a given protein, adding a FLAG-tag to a protein allows the protein to be studied with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis and Western blotting.
Purification Method:
Affinity purification
Storage Buffer:
Store at 4℃. Avoid freeze / thaw cycles.Buffer: 0.03% sodium azide,20% ethanol
Immunoprecipitation of GSK3B-Flag in 200 µg extracts from 293T cells transfected with GSK3B-Flag using 40 µl Anti-DDDDK (Nanobody) Agarose Beads mAb (AE125). Western blot analysis was performed using HRP-conjugated Rabbit anti DDDDK-Tag mAb (AE095) at 1:5000 dilution.
