Description
Anti-GFP VHH Magnetic Beads (CABE074)
The Anti-GFP VHH Magnetic Beads (CABE074) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, conjugated to agarose beads, allows for easy purification and immunoprecipitation of GFP-tagged proteins from various samples.Using a single domain antibody derived from llama, the CABE074 antibody is highly specific and efficient in capturing GFP-tagged proteins for downstream analysis. The agarose beads conjugated to the antibody provide a solid support matrix for easy handling and separation of the target protein.
This antibody is validated for use in IP, ChIP, RIP, CoIP applications and has demonstrated reactivity against Species independent samples.
| Product Name: | Anti-GFP VHH Magnetic Beads |
| SKU: | CABE074 |
| Size: | 20μL, 50μL, 100μL, 200μL |
| Reactivity: | Species independent |
| Conjugate: | Agarose Beads |
| Immunogen: | Recombinant protein (or fragment).This information is considered to be commercially sensitive. | ||||||||
| Tested Applications: | IP ChIP RIP CoIP | ||||||||
| Recommended Dilution: |
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| Synonyms: | GFP, GFP tag, GFP-tag |
| Calculated MW: | 27kDa |
| Observed MW: | 30kDa |
The green fluorescent protein (GFP) is a protein composed of 238 amino acid residues (26.9 kDa) that exhibits bright green fluorescence when exposed to light in the blue to ultraviolet range. Although many other marine organisms have similar green fluorescent proteins, GFP traditionally refers to the protein first isolated from the jellyfish Aequorea victoria. The GFP from A. victoria has a major excitation peak at a wavelength of 395 nm and a minor one at 475 nm. Its emission peak is at 509 nm, which is in the lower green portion of the visible spectrum. The GFP from the sea pansy (Renilla reniformis) has a single major excitation peak at 498 nm. GFP makes for an excellent tool in many forms of biology due to its ability to form internal chromophore without requiring any accessory cofactors, gene products, or enzymes / substrates other than molecular oxygen.In cell and molecular biology, the GFP gene is frequently used as a reporter of expression. It has been used in modified forms to make biosensors, and many animals have been created that express GFP, which demonstrates a proof of concept that a gene can be expressed throughout a given organism, in selected organs, or in cells of interest. GFP can be introduced into animals or other species through transgenic techniques, and maintained in their genome and that of their offspring. To date, GFP has been expressed in many species, including bacteria, yeasts, fungi, fish and mammals, including in human cells.
| Purification Method | Affinity purification |
| RRID | AB_2863798 |
| Buffer Information | Store at 4℃. Avoid freeze / thaw cycles. Buffer: 0.03% sodium azide,20% ethanol |
 | Immunoprecipitation analysis of 100 μg extracts of Eukaryotic expression of GFP using 30ul Anti-GFP(Nanobody) Agarose Beads antibody (CABE074). Western blot was performed from the immunoprecipitate using Mouse anti GFP-Tag mAb (AE012) at a dilution of 1:10000 |
