The AK4 Antibody (CAB13461) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific to human samples and has been validated for use in Western blotting applications. By binding to the AK4 protein, this antibody enables precise detection and analysis of AK4 expression in a variety of cell types, making it an ideal choice for studies in biochemistry and cell biology.AK4, also known as adenylate kinase 4, plays a crucial role in maintaining cellular energy balance by catalyzing the conversion of adenylate nucleotides.
This antibody is validated for use in WB, IP, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
AK4 Antibody
SKU:
CAB13461
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
This gene encodes a member of the adenylate kinase family of enzymes. The encoded protein is localized to the mitochondrial matrix. Adenylate kinases regulate the adenine and guanine nucleotide compositions within a cell by catalyzing the reversible transfer of phosphate group among these nucleotides. Five isozymes of adenylate kinase have been identified in vertebrates. Expression of these isozymes is tissue-specific and developmentally regulated. A pseudogene for this gene has been located on chromosome 17. Three transcript variants encoding the same protein have been identified for this gene. Sequence alignment suggests that the gene defined by NM_013410, NM_203464, and NM_001005353 is located on chromosome 1.
Purification Method
Affinity purification
Gene ID
205
RRID
AB_2760322
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using AK4 Rabbit pAb (CAB13461) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Immunoprecipitation analysis of 200 μg extracts of HepG2 cells, using 3 μg AK4 antibody (CAB13461). Western blot was performed from the immunoprecipitate using AK4 antibody (CAB13461) at a dilution of 1:1000.