The AKT1S1/PRAS40 Monoclonal Antibody (CAB3391) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, developed using advanced monoclonal antibody technology, specifically targets the AKT1S1 protein, allowing for precise detection and analysis in a variety of experimental settings.AKT1S1, also known as PRAS40, is a critical regulator of cell growth, proliferation, and survival, making it a key player in processes like tumorigenesis and metabolic regulation. By targeting the AKT1S1 protein, researchers can gain valuable insights into the mechanisms underlying these processes and potential therapeutic targets for diseases like cancer and metabolic disorders.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
AKT1S1/PRAS40 Monoclonal Antibody
SKU:
CAB3391
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC1965
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
Lobe, PRAS40, AKT1S1/PRAS40
Positive Sample:
HeLa, RD, MEF, Mouse ovary, Rat brain
Cellular Localization:
Cytoplasm, Cytosol.
Calculated MW:
27kDa
Observed MW:
40kDa
AKT1S1 is a proline-rich substrate of AKT (MIM 164730) that binds 14-3-3 protein (see YWHAH, MIM 113508) when phosphorylated (Kovacina et al., 2003 [PubMed 12524439]).
Purification Method
Affinity purification
Gene ID
84335
RRID
AB_2863050
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using AKT1S1/PRAS40 Rabbit mAb (CAB3391) at 1:1000 dilution incubated at room temperature for 1.5 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 20 s.
