The AKT1S1/PRAS40 Polyclonal Antibody (CAB21686) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits high specificity and reactivity with human samples, making it ideal for Western blot applications.AKT1S1/PRAS40, a known substrate of AKT, functions as a key regulator of mTORC1 activity, influencing cell growth and protein synthesis. Dysregulation of the AKT1S1/PRAS40 pathway has been implicated in various diseases, including cancer, diabetes, and neurological disorders, making it a promising target for therapeutic intervention.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
AKT1S1/PRAS40 Polyclonal Antibody
SKU:
CAB21686
Size:
20μL, 100μL
Reactivity:
Human
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
Lobe, PRAS40, AKT1S1/PRAS40
Positive Sample:
PC-3
Cellular Localization:
Cytoplasm, Cytosol.
Calculated MW:
27kDa
Observed MW:
40kDa
AKT1S1 is a proline-rich substrate of AKT (MIM 164730) that binds 14-3-3 protein (see YWHAH, MIM 113508) when phosphorylated (Kovacina et al., 2003 [PubMed 12524439]).
Purification Method
Affinity purification
Gene ID
84335
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from PC-3 cells using AKT1S1/PRAS40 Rabbit pAb (CAB21686) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 5s.
