The AKT1S1/PRAS40 Polyclonal Antibody (CAB6238) is a high-quality antibody developed for reliable detection and analysis of target proteins. AKT1S1 is a proline-rich substrate of AKT (MIM 164730) that binds 14-3-3 protein (see YWHAH, MIM 113508) when phosphorylated (Kovacina et al., 2003 [PubMed 12524439]). RRID AB_2766846 Gene ID 84335 Swiss Prot Synonym Lobe; PRAS40; AKT1S1/PRAS40
This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
AKT1S1/PRAS40 Polyclonal Antibody
SKU:
CAB6238
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Clone Number:
-
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBIHC-PIF/ICCELISA
Recommended Dilution:
WB
1:500 - 1:2000
IHC-P
1:50 - 1:100
IF
/
ICC
1:50 - 1:100
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
Lobe, PRAS40, AKT1S1/PRAS40
Positive Sample:
Mouse heart, Rat heart
Cellular Localization:
Cytoplasm, Cytosol.
Calculated MW:
27kDa
Observed MW:
50kDa
AKT1S1 is a proline-rich substrate of AKT (MIM 164730) that binds 14-3-3 protein (see YWHAH, MIM 113508) when phosphorylated (Kovacina et al., 2003 [PubMed 12524439]). RRID AB_2766846 Gene ID 84335 Swiss Prot Synonym Lobe; PRAS40; AKT1S1/PRAS40
Purification Method:
Affinity purification
Gene ID:
84335
RRID:
AB_2766846
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using AKT1S1/PRAS40 Rabbit pAb (CAB6238) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 90s.
Immunohistochemistry analysis of paraffin-embedded Human mammary cancer using AKT1S1/PRAS40 Rabbit pAb (CAB6238) at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunofluorescence analysis of NIH-3T3 cells using AKT1S1/PRAS40 Rabbit pAb (CAB6238) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
