The Aldosterone ELISA Kit is specifically designed for the precise measurement of aldosterone levels in serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides reliable and reproducible results, making it suitable for a variety of research applications.Aldosterone is a vital hormone that regulates electrolyte balance and blood pressure by acting on the kidneys to retain sodium and water while excreting potassium.
Imbalances in aldosterone levels can lead to conditions such as hypertension, heart failure, and kidney disease, making it a crucial biomarker for studying these diseases and evaluating treatment effectiveness.Overall, the Aldosterone ELISA Kit offers researchers a valuable tool for accurately quantifying aldosterone levels, contributing to a better understanding of its role in health and disease.
Product Name:
ALD (Aldosterone) ELISA Kit
SKU:
UNES00045
Target:
ALD (Aldosterone) (UNES00045)
Size:
96T
Assay type:
Competitive-ELISA
Assay time:
2.0h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with ALD. During the reaction, ALD in the sample or standard competes with a fixed amount of ALD on the solid phase supporter for sites on the Biotinylated Detection Ab specific to ALD. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of ALD in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
100-113
91-106
88-101
Average (%)
105
98
95
1:4
Range (%)
94-107
87-103
87-98
Average (%)
101
95
92
1:8
Range (%)
101-117
95-106
85-98
Average (%)
107
100
91
1:16
Range (%)
97-109
87-101
89-99
Average (%)
104
94
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-98
91
EDTA plasma (n=5)
96-112
102
Cell culture media (n=5)
90-102
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
51.8
89.6
476.9
46.8
94.2
479.4
Standard deviation
2.7
4.3
20.0
3.2
5.7
23.0
C V (%)
5.21
4.8
4.19
6.84
6.05
4.8
Sample type &Sample volume:
serum, plasma and other biological fluids; 50μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of ALD concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes ALD in samples. No significant cross-reactivity or interference between ALD and analogues was observed.
