The ANAPC4 Polyclonal Antibody (PAC018571) is a valuable tool for researchers studying the ANAPC4 protein, a key component of the Anaphase Promoting Complex/Cyclosome (APC/C) involved in cell cycle regulation. This antibody, generated in rabbits, exhibits high reactivity with human samples and is optimized for use in Western blot applications.ANAPC4 is essential for the proper function of the APC/C complex, which controls the progression of the cell cycle by targeting specific proteins for degradation. Dysregulation of this complex can lead to cell cycle abnormalities and is associated with various diseases, including cancer.
By targeting the ANAPC4 protein, researchers can investigate its role in cell cycle regulation and potentially develop new therapies that target the APC/C complex in diseases where cell proliferation is a factor. This antibody provides a valuable tool for understanding the mechanisms underlying cell cycle control and could lead to significant advancements in cancer research and drug discovery.
Antibody Name:
ANAPC4 Antibody (PACO18571)
Antibody SKU:
PACO18571
Size:
50ul
Host Species:
Rabbit
Tested Applications:
ELISA, IHC
Recommended Dilutions:
ELISA:1:2000-1:5000, IHC:1:25-1:100
Species Reactivity:
Human, Mouse
Immunogen:
Synthetic peptide of human ANAPC4
Form:
Liquid
Storage Buffer:
-20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Purification Method:
Antigen affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
The image on the left is immunohistochemistry of paraffin-embedded Human gastric cancer tissue using PACO18571(ANAPC4 Antibody) at dilution 1/30, on the right is treated with synthetic peptide. (Original magnification: x200).
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using PACO18571(ANAPC4 Antibody) at dilution 1/30, on the right is treated with synthetic peptide. (Original magnification: x200).
Background:
A large protein complex, termed the anaphase-promoting complex (APC), or the cyclosome, promotes metaphase-anaphase transition by ubiquitinating its specific substrates such as mitotic cyclins and anaphase inhibitor, which are subsequently degraded by the 26S proteasome. Biochemical studies have shown that the vertebrate APC contains eight subunits. The composition of the APC is highly conserved in organisms from yeast to humans. The exact function of this gene product is not known. Two transcript variants encoding different isoforms have been found for this gene.
Synonyms:
anaphase promoting complex subunit 4
UniProt Protein Function:
APC4: Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. Belongs to the APC4 family. 2 isoforms of the human protein are produced by alternative splicing.
Molecular Function:protein binding; ubiquitin-protein ligase activity; protein phosphatase binding
Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; mitosis; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; cell division; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; mitotic cell cycle spindle assembly checkpoint; mitotic cell cycle
NCBI Summary:
A large protein complex, termed the anaphase-promoting complex (APC), or the cyclosome, promotes metaphase-anaphase transition by ubiquitinating its specific substrates such as mitotic cyclins and anaphase inhibitor, which are subsequently degraded by the 26S proteasome. Biochemical studies have shown that the vertebrate APC contains eight subunits. The composition of the APC is highly conserved in organisms from yeast to humans. The exact function of this gene product is not known. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2013]
