The Acetyl-Histone H3-K23 Monoclonal Antibody (CAB2771) is a high-quality antibody developed for reliable detection and analysis of target proteins. Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. RRID Gene ID 8290 8350 Swiss Prot Synonym
This antibody is validated for use in WB, IHC-P, IF/ICC, IP, ChIP, ELISA, DB applications and has demonstrated reactivity against Human, Mouse, Rat, Other (Wide Range Predicted) samples.
Product Name:
Acetyl-Histone H3-K23 Monoclonal Antibody
SKU:
CAB2771
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat, Other (Wide Range Predicted)
Clone Number:
ARC53670
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBIHC-PIF/ICCIPChIPELISADB
Recommended Dilution:
WB
1:10000 - 1:120000
DB
1:500 - 1:2000
IHC-P
1:500 - 1:1000
IF
/
ICC
1:50 - 1:200
IP
0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements. Ch
IP
2μg antibody for 5μg-10μg of Chromatin
Synonyms:
-
Positive Sample:
HeLa treated with TSA, NIH/3T3 treated with TSA, C2C12 treated with TSA,
Cellular Localization:
Chromosome, Nucleus.
Calculated MW:
15kDa
Observed MW:
17kDa
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. RRID Gene ID 8290 8350 Swiss Prot Synonym
Purification Method:
Affinity purification
Gene ID:
8290 8350
RRID:
-
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from C2C12 cells using Acetyl-Histone H3-K27 Rabbit mAb (CAB2771) at 1:100000 dilution. C2C12 cells were treated with TSA (1 uM) at 37℃ for 18 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 20 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Dot-blot analysis of all sorts of peptides using Acetyl-Histone H3-K27 Rabbit mAb (CAB2771) at 1:200000 dilution.
Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer tissue using Acetyl-Histone H3-K27 Rabbit mAb (CAB2771) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human small intestine tissue using Acetyl-Histone H3-K27 Rabbit mAb (CAB2771) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human liver tissue using Acetyl-Histone H3-K27 Rabbit mAb (CAB2771) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using Acetyl-Histone H3-K27 Rabbit mAb (CAB2771) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse colon tissue using Acetyl-Histone H3-K27 Rabbit mAb (CAB2771) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunofluorescence analysis of HeLa treated with TSA and HeLa cells using Acetyl-Histone H3-K27 Rabbit mAb (CAB2771) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 600 μg extracts of HeLa cells treated with TSA using 5 μg Acetyl-Histone H3-K27 Rabbit mAb(CAB2771). Western blot was performed from the immunoprecipitate using Acetyl-Histone H3-K27 antibody (CAB2771) at a dilution of 1:50000.
Chromatin immunoprecipitation analysis of extracts of HeLa cells, using Acetyl-Histone H3-K27 antibody (CAB2771) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
