The alpha-Actinin-4 Monoclonal Antibody (CAB3379) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised against alpha-actinin-4, a cytoskeletal protein involved in cell motility and adhesion, is highly specific and sensitive for detecting alpha-actinin-4 in human samples. Its validated use in Western blot applications allows for precise detection and analysis of alpha-actinin-4 levels in various cell types.Alpha-actinin-4 is an important regulator of actin cytoskeleton dynamics, playing a key role in maintaining cell shape and movement. Dysregulation of alpha-actinin-4 has been implicated in various diseases, including cancer and kidney disorders, making it a target of interest for researchers in these fields.
This antibody is validated for use in WB, IF/ICC, IP, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
alpha-Actinin-4 Monoclonal Antibody
SKU:
CAB3379
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC1959
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
FSGS, FSGS1, ACTININ-4, α-Actinin-4
Positive Sample:
HeLa, 293T, Jurkat, Mouse brain, Mouse spleen, Rat lung
Cellular Localization:
Cell Junction, Cytoplasm, Nucleus.
Calculated MW:
105kDa
Observed MW:
100kDa
Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, alpha actinin isoform which is concentrated in the cytoplasm, and thought to be involved in metastatic processes. Mutations in this gene have been associated with focal and segmental glomerulosclerosis.
Purification Method
Affinity purification
Gene ID
81
RRID
AB_2863046
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using α-Actinin-4 Rabbit mAb (CAB3379) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Confocal imaging of U-2 OS cells using α-Actinin-4 Rabbit mAb (CAB3379, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (CABS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (CABS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
