The ATAD2 Monoclonal Antibody (CAB19792) is a high-quality antibody developed for reliable detection and analysis of target proteins. A large family of ATPases has been described, whose key feature is that they share a conserved region of about 220 amino acids that contains an ATP-binding site. The proteins that belong to this family either contain one or two AAA (ATPases Associated with diverse cellular Activities) domains. AAA family proteins often perform chaperone-like functions that assist in the assembly, operation, or disassembly of protein complexes. The protein encoded by this gene contains two AAA domains, as well as a bromodomain. RRID Gene ID 29028 Swiss Prot Synonym ANCCA; CT137; PRO2000; ATAD2
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
ATAD2 Monoclonal Antibody
SKU:
CAB19792
Size:
100μL, 20μL
Reactivity:
Human
Clone Number:
ARC2317
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:2000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
ANCCA, CT137, PRO2000, ATAD2
Positive Sample:
MCF7, K-562
Cellular Localization:
Extracellular Exosome, Nucleoplasm, Nucleus.
Calculated MW:
159kDa
Observed MW:
180kDa
A large family of ATPases has been described, whose key feature is that they share a conserved region of about 220 amino acids that contains an ATP-binding site. The proteins that belong to this family either contain one or two AAA (ATPases Associated with diverse cellular Activities) domains. AAA family proteins often perform chaperone-like functions that assist in the assembly, operation, or disassembly of protein complexes. The protein encoded by this gene contains two AAA domains, as well as a bromodomain. RRID Gene ID 29028 Swiss Prot Synonym ANCCA; CT137; PRO2000; ATAD2
Purification Method:
Affinity purification
Gene ID:
29028
RRID:
-
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using ATAD2 Rabbit mAb (CAB19792) at 1:1000 dilution incubated overnight at 4°C. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
