The ATRIP Monoclonal Antibody (CAB19279) is a high-quality antibody developed for reliable detection and analysis of target proteins. This polyclonal antibody, produced in rabbits, exhibits high specificity and sensitivity towards human ATRIP, making it suitable for Western blot applications. By binding to ATRIP, this antibody allows for the detection and analysis of ATRIP levels in various cell types, facilitating investigations into DNA damage response mechanisms.ATRIP is essential for activating the ATR kinase in response to DNA damage, playing a key role in maintaining genomic stability and preventing the accumulation of mutations that can lead to cancer and other diseases.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
ATRIP Monoclonal Antibody
SKU:
CAB19279
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC2460
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
ATRIP, ATR-interacting protein
Positive Sample:
MCF7, Jurkat, Mouse testis, Mouse brain, Rat testis, Rat brain
Cellular Localization:
Nucleus.
Calculated MW:
86kDa
Observed MW:
90kDa
This gene encodes an essential component of the DNA damage checkpoint. The encoded protein binds to single-stranded DNA coated with replication protein A. The protein also interacts with the ataxia telangiectasia and Rad3 related protein kinase, resulting in its accumulation at intranuclear foci induced by DNA damage. Multiple transcript variants encoding different isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
84126
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using ATRIP Rabbit mAb (CAB19279) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
