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Bcdin3d Polyclonal Antibody (CAB20720)

SKU:
CAB20720
Product Type:
Antibody
Reactivity:
Human
Mouse
Host Species:
Rabbit
Isotype:
IgG
  • Immunofluorescence analysis of NIH/3T3 cells using Bcdin3d Rabbit pAb at dilution of 1:25 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of MCF7 cells, using Bcdin3d antibody at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
$167 - $503
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Description

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Product Name:Bcdin3d Polyclonal Antibody
SKU:CAB20720
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 65-197 of mouse Bcdin3d (NP_083512.2).
Sequence:EKRP ILGL DVGC NSGD LSVA LYKH FLSP RDGE TCSG ASRE LRIL CCDI DPVL VERA ERDC PFPE ALTF ITLD IMDQ ESRK VPLS SFLS QFGR SVFD MVFC MSVT MWIH LNHG DRGL CEFL AHVS SLCS YLLV E
Tested Applications:WB IF/ICC ELISA
Recommended Dilution:WB,1:100 - 1:500 IF/ICC,1:50 - 1:200
Synonyms:4930556P03Rik; Bcdin3d
Positive Sample:MCF7
Conjugate:Unconjugated
Cellular Localization:cytoplasm, cytosol, nucleoplasm
Calculated MW:32kDa
Observed MW:33kDa

Predicted to enable O-methyltransferase activity and tRNA methyltransferase activity. Predicted to be involved in miRNA 2'-O-methylation; negative regulation of pre-miRNA processing; and tRNA methylation. Predicted to act upstream of or within methylation. Predicted to be located in cytosol and nucleoplasm. Predicted to be active in cytoplasm. Orthologous to human BCDIN3D (BCDIN3 domain containing RNA methyltransferase).

Purification Method:Affinity purification
Gene ID:75284
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Product ImageWestern blot analysis of extracts of MCF7 cells, using Bcdin3d antibody (CAB20720) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.

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