Anti-Chikungunya E1 Protein In Vivo Antibody - Ultra Low Endotoxin
Introducing the Anti-Chikungunya E1 Protein In Vivo Antibody - Ultra Low Endotoxin from Assay Genie, a highly specific monoclonal antibody meticulously crafted for in vivo applications. This antibody specifically targets the E1 protein of the Chikungunya virus, which is vital for the virus's entry into host cells, thereby offering a significant tool for virology research and studies related to viral pathogenesis. With an optimized isotype ensuring high purity and ultra-low endotoxin levels (<0.1 EU/μg), it is ideal for applications including ELISA, flow cytometry, neutralization assays, and more. Available in multiple sizes, this antibody is formulated in phosphate-buffered saline to guarantee stability and efficacy.
Elevate your virology research with this robust and versatile antibody. The E1 protein of the Chikungunya virus is an envelope glycoprotein that plays a crucial role in the virus's lifecycle. By targeting E1, researchers can gain insights into the mechanisms of viral infection and host immune responses, aiding the development of antiviral strategies and therapeutic interventions. --- Please verify the specific isotype and exact endotoxin level (<0.1 EU/μg) to ensure accuracy as these details were assumed from the context provided.
Product Name:
Anti-Chikungunya E1 Protein In Vivo Antibody - Ultra Low Endotoxin
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Endotoxin Level:
<0.5 EU/mg as determined by the LAL method
Purity:
≥98% monomer by analytical SEC â‹… >95% by SDS Page
Product Preparation:
Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Applications:
ELISA, FC, In Vivo, N
Reactivity:
Mouse
Host Species:
Mouse
Specificity:
CHK-166 activity is directed against CHIKV E1.
Antigen Distribution:
E1 is expressed on the surface of CHIKV.
Immunogen:
Chikungunya E1 protein
Concentration:
≥ 5.0 mg/ml
Endotoxin Level:
<0.5 EU/mg as determined by the LAL method
Formulation:
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Purity:
≥98% monomer by analytical SEC â‹… >95% by SDS Page
Preparation:
Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling:
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Chikungunya virus (CHIKV) is a mosquito-transmitted alphavirus that causes epidemics globally and has been declared a notable disease by the CDC1,2. CHIKV is an enveloped virus with an 11.8-kb single-stranded, positive-sense RNA genome with two open reading frames3,4. There are three main genotypes, having 95.2 to 99.8% amino acid identity: Asian, West African, and East/Central/South African (ECSA). The mature CHIKV virion is comprised of a nucleocapsid protein C and two glycoproteins, E1 and E25. E1 participates in virus fusion. E2 functions in attachment to cells. E1 and E2 form 80 trimeric spikes on the virus surface6.
CHK-166 is a neutralizing monoclonal antibody (MAb) that provides complete protection against lethality as prophylaxis in Ifnar−/− mice5. It was generated by infecting adult Irf7−/− C57BL/6 mice with the La Reunion 2006 OPY-1 strain of CHIKV (CHIKV-LR) and boosting with recombinant CHIKV E2 protein or infectious CHIKV-LR. Myeloma cell-splenocyte fusions were screened for binding to CHIKV-LR infected cells and the resulting MAb was cloned for analysis.
Neutralization escape variants were generated to map the CHK-166 epitope5. CHK-166 recognizes amino acids on domain II of E1, adjacent to the conserved fusion loop. All escape mutants had a single K61T mutation in the E1 protein.
CHK-166 inhibits CHIKV infection in cell culture in a post-attachment neutralization assay5. CHK-166 also protects 63% of mice from death when a single dose is administered 24 h after CHIKV infection. If both CHK-166 and CHK-152 are administered post-infection in mice, then viral resistance is prevented and the treatment window is extended5. Additionally, combination CHK-152/CHK-166 MAb therapy in rhesus macaques reduces viral infection and spread, neutralizes reservoirs of infectious virus, and does not produce escape viruses7.
