The Chromogranin B Monoclonal Antibody (CAB3517) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, is highly specific for human samples and is validated for use in various applications, including immunohistochemistry and ELISA. By binding to Chromogranin B, the antibody allows for the detection and analysis of this protein in different cell types, making it an ideal choice for studies in endocrinology and neuroscience.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Rat samples.
Product Name:
Chromogranin B Monoclonal Antibody
SKU:
CAB3517
Size:
20μL, 100μL
Reactivity:
Human, Rat
Clone Number:
ARC2023
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
SCG1, Chromogranin B
Positive Sample:
293T, PC-12, BxPC-3, SH-SY5Y
Cellular Localization:
Endoplasmic Reticulum Lumen, Extracellular Space.
Calculated MW:
78kDa
Observed MW:
110kDa
This gene encodes a tyrosine-sulfated secretory protein abundant in peptidergic endocrine cells and neurons. This protein may serve as a precursor for regulatory peptides.
Purification Method
Affinity purification
Gene ID
1114
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Chromogranin B Rabbit mAb (CAB3517) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
