The DMAP1 Monoclonal Antibody (CAB19281) is a high-quality antibody developed for reliable detection and analysis of target proteins. This gene encodes a subunit of several, distinct complexes involved in the repression or activation of transcription. The encoded protein can independently repress transcription and is targeted to replication foci throughout S phase by interacting directly with the N-terminus of DNA methyltransferase 1. During late S phase, histone deacetylase 2 is added to this complex, providing a means to deacetylate histones in transcriptionally inactive heterochromatin following replication. The encoded protein is also a component of the nucleosome acetyltransferase of H4 complex and interacts with the transcriptional corepressor tumor susceptibility gene 101 and the pro-apoptotic death-associated protein 6, among others. Alternatively spliced transcript variants encoding the same protein have been described. RRID Gene ID 55929 Swiss Prot Synonym EAF2; SWC4; MEAF2; DNMAP1; DNMTAP1; DMAP1
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
DMAP1 Monoclonal Antibody
SKU:
CAB19281
Size:
100μL, 20μL
Reactivity:
Human
Clone Number:
ARC2462
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:1000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
EAF2, SWC4, MEAF2, DNMAP1, DNMTAP1, DMAP1
Positive Sample:
HeLa, PC-12, HepG2
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
53kDa
Observed MW:
60kDa
This gene encodes a subunit of several, distinct complexes involved in the repression or activation of transcription. The encoded protein can independently repress transcription and is targeted to replication foci throughout S phase by interacting directly with the N-terminus of DNA methyltransferase 1. During late S phase, histone deacetylase 2 is added to this complex, providing a means to deacetylate histones in transcriptionally inactive heterochromatin following replication. The encoded protein is also a component of the nucleosome acetyltransferase of H4 complex and interacts with the transcriptional corepressor tumor susceptibility gene 101 and the pro-apoptotic death-associated protein 6, among others. Alternatively spliced transcript variants encoding the same protein have been described. RRID Gene ID 55929 Swiss Prot Synonym EAF2; SWC4; MEAF2; DNMAP1; DNMTAP1; DMAP1
Purification Method:
Affinity purification
Gene ID:
55929
RRID:
-
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using DMAP1 Rabbit mAb (CAB19281) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
