The Engrailed 1 Monoclonal Antibody (CAB19672) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, has been validated for use in various applications including Western blot and immunohistochemistry. It binds specifically to the Engrailed-1 protein, allowing for precise detection and analysis in different cell types and tissues.Engrailed-1 is a transcription factor that regulates gene expression and is essential for the development of the central nervous system. Dysregulation of Engrailed-1 has been implicated in various neurological disorders, making it a valuable target for studies in neuroscience and developmental biology.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Mouse, Rat samples.
Product Name:
Engrailed 1 Monoclonal Antibody
SKU:
CAB19672
Size:
20μL, 100μL
Reactivity:
Mouse, Rat
Clone Number:
ARC2225
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
ENDOVESLB, Engrailed 1
Positive Sample:
Mouse brain, Rat brain
Cellular Localization:
Nucleus.
Calculated MW:
40kDa
Observed MW:
50kDa
Homeobox-containing genes are thought to have a role in controlling development. In Drosophila, the 'engrailed' (en) gene plays an important role during development in segmentation, where it is required for the formation of posterior compartments. Different mutations in the mouse homologs, En1 and En2, produced different developmental defects that frequently are lethal. The human engrailed homologs 1 and 2 encode homeodomain-containing proteins and have been implicated in the control of pattern formation during development of the central nervous system.
Purification Method
Affinity purification
Gene ID
2019
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Engrailed 1 Rabbit mAb (CAB19672) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
