The Fluorescein FITC Monoclonal Antibody (CAB20797) is a high-quality antibody developed for reliable detection and analysis of target proteins. Fluorescein isothiocyanate (FITC) labeling is a common technique with a wide range of? applications because it reacts quickly with amines and due to its high quantum efficacy. Due to its high molecular absorptivity, using FITC labels is preferred over conventional colorimetric labels and radio labels because fluorophores like FITC are bright, easier to work with, and don’t require special waste handling. Proteins, substrates, peptide hormones, and antibodies? labeled by FITC can be used as probes in flow cytometry, enzyme kinetics, and immunocytochemistry, as well as in the detection of receptors on the surface of? the target cells.5-FAM is the purified single isomer of carboxyfluorescein. It is one of the most popular green fluorescent reagents used for labeling peptides, proteins and nucleotides. It has been predominantly used to develop a variety of green fluorescent peptides that can be excited with the 488 nm line of the Ar laser. It has also been used to prepare various small fluorescent molecules.6-FAM (6-Carboxyfluorescein) contains a carboxylic acid that can be used to react with primary amines via carbodiimide activation of the carboxylic acid. Fluorescein is the most common fluorescent derivatization reagent for labeling biomolecules. In addition to its relatively high absorptivity, excellent fluorescence quantum yield, and good water solubility, fluorescein has an excitation maximum that closely matches the 488 nm spectral line of the argon-ion laser.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Species independent samples.
Product Name:
Fluorescein FITC Monoclonal Antibody
SKU:
CAB20797
Size:
100μL, 20μL
Reactivity:
Species independent
Clone Number:
ARC51071
Conjugate:
Unconjugated
Immunogen:
Chemical compounds corresponding to FITC/5-FAM/6-FAM.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:2000 - 1:12000
ELISA
1:500 - 1:2000
Observed MW:
25kDa/55kDa
Fluorescein isothiocyanate (FITC) labeling is a common technique with a wide range of? applications because it reacts quickly with amines and due to its high quantum efficacy. Due to its high molecular absorptivity, using FITC labels is preferred over conventional colorimetric labels and radio labels because fluorophores like FITC are bright, easier to work with, and don’t require special waste handling. Proteins, substrates, peptide hormones, and antibodies? labeled by FITC can be used as probes in flow cytometry, enzyme kinetics, and immunocytochemistry, as well as in the detection of receptors on the surface of? the target cells.5-FAM is the purified single isomer of carboxyfluorescein. It is one of the most popular green fluorescent reagents used for labeling peptides, proteins and nucleotides. It has been predominantly used to develop a variety of green fluorescent peptides that can be excited with the 488 nm line of the Ar laser. It has also been used to prepare various small fluorescent molecules.6-FAM (6-Carboxyfluorescein) contains a carboxylic acid that can be used to react with primary amines via carbodiimide activation of the carboxylic acid. Fluorescein is the most common fluorescent derivatization reagent for labeling biomolecules. In addition to its relatively high absorptivity, excellent fluorescence quantum yield, and good water solubility, fluorescein has an excitation maximum that closely matches the 488 nm spectral line of the argon-ion laser.
Purification Method
Affinity purification
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of recombinant FITC-conjugated goat IgG protein using FITC/5-FAM/6-FAM Rabbit mAb (CAB20797) at 1:10000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 100 ng/50 ng/10 ng per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Immobilized biotin-6 Fam, biotin-5 Fam, biotin-FITC, at 1 μg/mL (25 μl/well), of which EC50 ranges are 0.27-0.54 ng/mL, 0.18-0.31 ng/mL and 0.12-0.39 ng/mL , can bind anti 6-Fam/ 5-Fam/ FITC mAb (CAB20797), respectively.
