The Fluorescein Isothiocyanate/FITC Antibody (CAB20385) is a high-quality antibody developed for reliable detection and analysis of target proteins. Fluorescein isothiocyanate (FITC) is a derivative of fluorescein used in wide-ranging applications. It is the original fluorescein molecule functionalized with an isothiocyanate reactive group (-N=C=S), replacing a hydrogen atom on the bottom ring of the structure. FITC is a fluorochrome dye that absorbs ultraviolet or blue light causing molecules to become excited and emit a visible yellow-green light. FITC is a commonly used marker for antibodies in immunofluorescent techniques since the conjugation of FITC to proteins is relatively easy and does not, in general, destroy the biological activity of the labeled protein. FITC is also widely used as a hapten to label different proteins. RRID Gene ID Swiss Prot Synonym
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Species independent samples.
Product Name:
Fluorescein Isothiocyanate/FITC Antibody
SKU:
CAB20385
Size:
100μL, 20μL
Reactivity:
Species independent
Clone Number:
-
Conjugate:
Unconjugated
Immunogen:
Chemical compounds corresponding to Fluorescein Isothiocyanate/FITC.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:2000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
-
Positive Sample:
-
Cellular Localization:
-
Calculated MW:
-
Observed MW:
25kDa/55kDa
Fluorescein isothiocyanate (FITC) is a derivative of fluorescein used in wide-ranging applications. It is the original fluorescein molecule functionalized with an isothiocyanate reactive group (-N=C=S), replacing a hydrogen atom on the bottom ring of the structure. FITC is a fluorochrome dye that absorbs ultraviolet or blue light causing molecules to become excited and emit a visible yellow-green light. FITC is a commonly used marker for antibodies in immunofluorescent techniques since the conjugation of FITC to proteins is relatively easy and does not, in general, destroy the biological activity of the labeled protein. FITC is also widely used as a hapten to label different proteins. RRID Gene ID Swiss Prot Synonym
Purification Method:
Affinity purification
Gene ID:
-
RRID:
-
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
The FITC rabbit polyclonal antibody (CAB20385) are tested in Western blot against FITC conjugated Goat IgG,TRITC conjugated Goat IgG and unconjugated Goat IgG Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: Refer to figures. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
