Anti-VPS36 Antibody (CAB15157)
- SKU:
- CAB15157
- Product Type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-VPS36 Antibody |
| Antibody SKU: | CAB15157 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 70-220 of human VPS36 (NP_057159.2). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | Rat liver, Rat kidney |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 70-220 of human VPS36 (NP_057159.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | EEQA AGIG KSAK IVVH LHPA PPNK EPGP FQSS KNSY IKLS FKEH GQIE FYRR LSEE MTQR RWEN MPVS QSLQ TNRG PQPG RIRA VGIV GIER KLEE KRKE TDKN ISEA FEDL SKLM IKAK EMVE LSKS IANK IKDK QGDI TEDE TIRF KSY |
| Gene ID: | 51028 |
| Uniprot: | Q86VN1 |
| Cellular Location: | Cytoplasm, Endosome, Late endosome, Membrane, Nucleus |
| Calculated MW: | 36kDa/43kDa |
| Observed MW: | 43kDa |
| Synonyms: | VPS36, C13orf9, CGI-145, EAP45 |
| Background: | This gene encodes a protein that is a subunit of the endosomal sorting complex required for transport II (ESCRT-II). This protein complex functions in sorting of ubiquitinated membrane proteins during endocytosis. A similar protein complex in rat is associated with RNA polymerase elongation factor II. |
| UniProt Code: | Q86VN1 |
| NCBI GenInfo Identifier: | 71051598 |
| NCBI Gene ID: | 51028 |
| NCBI Accession: | NP_057159 |
| UniProt Secondary Accession: | Q86VN1,Q3ZCV7, Q5H9S1, Q5VXB6, Q9H8Z5, Q9Y3E3, A8K125 |
| UniProt Related Accession: | Q86VN1 |
| Molecular Weight: | 36,958 Da |
| NCBI Full Name: | vacuolar protein-sorting-associated protein 36 isoform 1 |
| NCBI Synonym Full Names: | vacuolar protein sorting 36 homolog (S. cerevisiae) |
| NCBI Official Symbol: | VPS36Â Â |
| NCBI Official Synonym Symbols: | EAP45; C13orf9; CGI-145Â Â |
| NCBI Protein Information: | vacuolar protein-sorting-associated protein 36; ELL-associated protein of 45 kDa; ESCRT-II complex subunit VPS36 |
| UniProt Protein Name: | Vacuolar protein-sorting-associated protein 36 |
| UniProt Synonym Protein Names: | ELL-associated protein of 45 kDa; ESCRT-II complex subunit VPS36 |
| Protein Family: | Vacuolar protein sorting-associated protein |
| UniProt Gene Name: | VPS36Â Â |
| UniProt Entry Name: | VPS36_HUMAN |
 | Western blot analysis of extracts of various cell lines, using VPS36 antibody (CAB15157) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunofluorescence analysis of NIH-3T3 cells using VPS36 Polyclonal Antibody (CAB15157) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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