The gamma-Tubulin Monoclonal Antibody (CAB9657) is a high-quality antibody developed for reliable detection and analysis of target proteins. This rabbit polyclonal antibody is highly specific to human gamma tubulin and has been validated for use in Western blot applications. By binding to gamma tubulin, this antibody allows for the detection and analysis of this important protein in various cell types.Gamma tubulin plays a crucial role in the regulation of microtubule dynamics and is essential for cell division and intracellular transport.
This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
gamma-Tubulin Monoclonal Antibody
SKU:
CAB9657
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC1684
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
This gene encodes a member of the tubulin superfamily. The encoded protein localizes to the centrosome where it binds to microtubules as part of a complex referred to as the gamma-tubulin ring complex. The protein mediates microtubule nucleation and is required for microtubule formation and progression of the cell cycle. A pseudogene of this gene is found on chromosome 7.
Purification Method
Affinity purification
Gene ID
7283
RRID
AB_2863751
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using γ-Tubulin Rabbit mAb (CAB9657) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3s.
Immunohistochemistry analysis of paraffin-embedded Rat testis using γ-Tubulin Rabbit mAb (CAB9657) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse testis using γ-Tubulin Rabbit mAb (CAB9657) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
Confocal imaging of NIH-3T3 cells using γ-Tubulin Rabbit mAb (CAB9657, dilution 1:100) (Red). The cells were counterstained with Alpha-tubulin (ubiquitous) chain Rabbit mAb (AC039, dilution 1:100) (Green). DAPI was used for nuclear staining (blue). Objective: 60x.
