The HES5 Monoclonal Antibody (CAB9768) is a high-quality antibody developed for reliable detection and analysis of target proteins. This gene encodes a member of a family of basic helix-loop-helix transcriptional repressors. The protein product of this gene, which is activated downstream of the Notch pathway, regulates cell differentiation in multiple tissues. Disruptions in the normal expression of this gene have been associated with developmental diseases and cancer. RRID AB_2863776 Gene ID 388585 Swiss Prot Synonym bHLHb38; HES5
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Mouse, Rat samples.
Product Name:
HES5 Monoclonal Antibody
SKU:
CAB9768
Size:
100μL, 20μL
Reactivity:
Mouse, Rat
Clone Number:
ARC1740
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBIHC-PELISA
Recommended Dilution:
WB
1:1000 - 1:6000
IHC-P
1:100 - 1:500
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
bHLHb38, HES5
Positive Sample:
Mouse brain, Mouse spleen, Rat brain, Rat spleen
Cellular Localization:
Nucleus.
Calculated MW:
18kDa
Observed MW:
18kDa
This gene encodes a member of a family of basic helix-loop-helix transcriptional repressors. The protein product of this gene, which is activated downstream of the Notch pathway, regulates cell differentiation in multiple tissues. Disruptions in the normal expression of this gene have been associated with developmental diseases and cancer. RRID AB_2863776 Gene ID 388585 Swiss Prot Synonym bHLHb38; HES5
Purification Method:
Affinity purification
Gene ID:
388585
RRID:
AB_2863776
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using HES5 Rabbit mAb (CAB9768) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Immunohistochemistry analysis of paraffin-embedded Rat testis tissue using HES5 Rabbit mAb (CAB9768) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
