The HSPA12A Monoclonal Antibody (CAB19825) is a high-quality antibody developed for reliable detection and analysis of target proteins. Predicted to enable ATP binding activity. Located in extracellular exosome.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
HSPA12A Monoclonal Antibody
SKU:
CAB19825
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC2351
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:1000 - 1:3000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
HSPA12A , HSPA12A
Positive Sample:
Mouse brain, Rat brain, SH-SY5Y
Cellular Localization:
Cytoplasm, Extracellular Exosome, Nucleus.
Calculated MW:
75 kDa
Observed MW:
75 kDa
Predicted to enable ATP binding activity. Located in extracellular exosome.
Purification Method
Affinity purification
Gene ID
259217
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using HSPA12A Rabbit mAb (CAB19825) at 1:3000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10 s.
Western blot analysis of various lysates using HSPA12A Rabbit mAb (CAB19825) at 1:1000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90 s.
