The HSPA12A Monoclonal Antibody (CAB19825) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, demonstrates high reactivity with human samples and has been validated for use in Western blot applications.HSPA12A, also known as a heat shock protein, is essential for maintaining proper protein folding and preventing cellular damage in times of stress. By binding to the HSPA12A protein, this antibody allows for accurate detection and analysis in a variety of cell types, making it ideal for studies in cell biology, molecular biology, and disease research.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
HSPA12A Monoclonal Antibody
SKU:
CAB19825
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC2351
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
HSPA12A , HSPA12A
Positive Sample:
Mouse brain, Rat brain, SH-SY5Y
Cellular Localization:
Cytoplasm, Extracellular Exosome, Nucleus.
Calculated MW:
75kDa
Observed MW:
75kDa
Predicted to enable ATP binding activity. Located in extracellular exosome.
Purification Method
Affinity purification
Gene ID
259217
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using HSPA12A Rabbit mAb (CAB19825) at 1:3000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10 s.
Western blot analysis of various lysates using HSPA12A Rabbit mAb (CAB19825) at 1:1000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90 s.
