The HSPA14 Monoclonal Antibody (CAB2286) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, developed in rabbits, exhibits strong reactivity with human samples and has been validated for use in Western blot experiments. By specifically targeting the HSPA14 protein, this antibody allows for accurate detection and analysis in a variety of cell types, making it ideal for investigations in the fields of cell biology and molecular biology.HSPA14, also known as a molecular chaperone, plays a vital role in maintaining protein homeostasis and facilitating proper protein folding under stressful conditions.
This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
HSPA14 Monoclonal Antibody
SKU:
CAB2286
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Clone Number:
ARC1908
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
HSP70-4, HSP70L1, MSANTD7, HSPA14
Positive Sample:
293T, HepG2, Mouse thymus
Cellular Localization:
Cytoplasm, Cytosol.
Calculated MW:
55kDa
Observed MW:
55kDa
Predicted to enable several functions, including ATP binding activity; misfolded protein binding activity; and unfolded protein binding activity. Predicted to be involved in several processes, including cellular response to unfolded protein; chaperone cofactor-dependent protein refolding; and protein refolding. Located in cytosol. Colocalizes with ribosome.
Purification Method
Affinity purification
Gene ID
51182
RRID
AB_2862989
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using HSPA14 Rabbit mAb (CAB2286) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Immunohistochemistry analysis of paraffin-embedded Human colon using HSPA14 Rabbit mAb (CAB2286) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
