![Anti-Human Hepsin [3H10.1] In Vivo Antibody - Low Endotoxin](https://cdn11.bigcommerce.com/s-h68l9z2lnx/images/stencil/1280x1280/products/214464/569460/anti-human-hepsin-3h10.1-in-vivo-antibody-low-endotoxin__22550.1673496990.jpg?c=2?imbypass=on "Anti-Human Hepsin [3H10.1] In Vivo Antibody - Low Endotoxin")
Description
Anti-Human Hepsin [3H10.1] In Vivo Antibody - Low Endotoxin
Introducing the Anti-Human Hepsin [3H10-1] In Vivo Antibody - Low Endotoxin from Assay Genie, a highly specific monoclonal antibody designed for in vivo applications. This antibody targets the Hepsin protein, which is significant in various cellular processes, making it ideal for research in oncology, cell biology, and related fields. With a mouse IgG1 isotype, it ensures high purity and low endotoxin levels (<1.0 EU/mg), perfect for applications such as ELISA, immunohistochemistry, and other assays. Available in various sizes, it is formulated in phosphate-buffered saline for stability and efficacy.
Enhance your research with this reliable and versatile antibody. Hepsin is a type II transmembrane serine protease primarily expressed in the liver and kidney, but also in various cancer cells. It plays an essential role in cell growth and maintenance, and its dysregulation is associated with tumor progression and metastasis. This makes Anti-Human Hepsin [3H10-1] In Vivo Antibody a valuable tool for researchers focused on understanding cancer mechanisms and developing potential therapeutic strategies.
| Product Name: | Anti-Human Hepsin [3H10.1] In Vivo Antibody - Low Endotoxin |
| Product Code: | IVMB0129 |
| Size: | 1mg, 5mg, 25mg, 50mg, 100mg |
| Clone: | 3H10.1 |
| Protein: | Hepsin |
| Product Type: | Monoclonal Antibody |
| Synonyms: | HPN, TMPRSS1, 3H10.1.2 |
| Isotype: | Mouse IgG1 κ |
| Reactivity: | Human |
| Immunogen: | Human hepsin protein |
| Applications: | ELISA, FC |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
| Endotoxin Level: | < 1.0 EU/mg as determined by the LAL method |
| Purity: | ≥95% monomer by analytical SEC >95% by SDS Page |
| Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Storage and Handling: | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. |
| Applications: | ELISA, FC |
| Recommended Usage: | ELISA FC Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. |
| Reactivity: | Human |
| Host Species: | Mouse |
| Specificity: | Clone 3H10.1.2 is able to recognize full-length native Hepsin expressed on the cell surface in addition to the recombinant soluble form. Clones 3H1.1.1 and 1F2.1.1 bind to the same epitope as clone 3H10.1.2 and inhibit it (and each other) from binding Hepsin. Clone 3H10.1.2 and clone 2D5.1.9 bind separate epitopes and do not inhibit each other from binding Hepsin. |
| Antigen Distribution: | Hepsin is expressed on the surface of epithelial cells including the liver, kidney, prostate, and thyroid in human tissues. |
| Immunogen: | Human hepsin protein |
| Concentration: | ≥ 5.0 mg/ml |
| Endotoxin Level: | < 1.0 EU/mg as determined by the LAL method |
| Purity: | ≥95% monomer by analytical SEC >95% by SDS Page |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
| Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Storage and Handling: | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. |
Hepsin is a type II transmembrane serine protease (TTSP) expressed on the surface of epithelial cells including the liver, kidney, prostate, and thyroid in human tissues. The physiological function of hepsin is unclear, although, In vitro studies have shown that hepsin activates blood clotting factors VII, XII, and IX, pro-urokinase (pro-uPA), and pro-hepatocyte growth factor (pro-HGF). The over-expression of hepsin has been implicated in several types of cancer, especially ovarian and prostate, which makes it an attractive diagnostic marker for cancers. Most notably, hepsin has been identified as one of the most highly induced genes in prostate cancer, and this over-expression is correlated with the cancer progression and metastasis. Furthermore anti-hepsin antibodies have been shown to inhibit the invasion of human prostate cancer cells.1
| Technical Datasheet: | View |
| Protein: | Hepsin |
| Research Area: | Immuno-Oncology |
| Mouse IgG1 Isotype Control [HKSP] | |
|---|---|
| Clone | HKSP |
| Isotype | Mouse IgG1 |
| Endotoxin Level | Low Endotoxin |
| Mouse IgG1 Isotype Control [HKSP84] | |
|---|---|
| Clone | HKSP84 |
| Isotype | Mouse IgG1 |
| Endotoxin Level | Low Endotoxin |
![Anti-Human Hepsin [3H10.1] In Vivo Antibody - Low Endotoxin](https://cdn11.bigcommerce.com/s-h68l9z2lnx/images/stencil/608x608/products/214464/569460/anti-human-hepsin-3h10.1-in-vivo-antibody-low-endotoxin__22550.1673496990.jpg?c=2 "Anti-Human Hepsin [3H10.1] In Vivo Antibody - Low Endotoxin")
